Raman · Gold Nanoprisms as Raman Signal Amplifiers for Bioimaging of Lung Cancer
FP7 — People (Marie Curie Actions)
- Duration
- 2013-04-01 → 2015-03-31
- EU contribution
- €254,638
- Participants
- 1
- Scheme
- MC-IEF
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Results in brief
Gold Nanoprisms as Raman Signal Amplifiers for Bioimaging of Lung Cancer
This project aimed to synthesis and characterise a range of biologically compatible gold nanoprisms for applications in Raman based disease diagnosis. The biocompatible gold nano particle were systematically synthesised in terms of different shape and dimensions and the resultant Surface Enhanced Raman signals (SERS) on small molecules and body fluids were optimised (Fig 1 and fig 2). Strategically designed PEGylated nanoparticles were developed with the aim of investigating the feasibility of using gold nano particles as a novel enhancement agent for Raman imaging of Lung adenocarcinoma cells. Toxicity tests of different shaped gold nanoparticle were analysed to compare the biocompatibility (Fig.3).
Data: CORDIS, © European Union
Project objective
Raman spectroscopy is a powerful tool for probing biochemical changes in situ at tissue level. The vibrational profile can provide invaluable molecular details for medical diagnosis and disease prognosis. Important technological as well as health and safety considerations, however, limits its clinical application, as spontaneous Raman is a relatively weak effect and high laser powers are required for rapid screening. Surface-enhanced Raman scattering (SERS) makes is possible to probe single molecules adsorbed onto a silver or gold nanoparticle, greatly enhancing the sensitivity of spontaneous Raman and therefore potentially significantly reducing screening times. However, the SERS sensitivity from spherical gold nanoparticles is still insufficient to detect trace biomolecules and/or the interaction between biomolecules, which may be overcome through using novel shape nanomaterials as SERS substrates for the improvement of the bio-detection limit. Recently, Pegylated gold nanoprisms (AuNPrs) have been successfully employed as signal amplifiers in multimodal in vivo imaging of cancer cells, and therefore pose a great potential for Raman spectroscopy as well. Excellent biocompatibility properties are known for Pegylated AuNPrs, with side lengths of 120nm and thicknesses of 10nm. They absorb strongly at a wavelength of 830 nm, with co-efficients of up to 37 µg.mL-1•OD-1, a wavelength within the tissue transparency window, promising deep penetration in vivo This project will explore the optimisation of Pegylated AuNPrs for surface enhancement of the Raman signal (SERS)of their local cellular environment for probing disease in vitro and ulimately in vivo. Lung cancer is the most common cause of death from cancer in the EU and is therefore chosen as a model for the development of a Raman based rapid screening technique for early detection of disease.
Original text from CORDIS.
Participants
- INSTITIUID TEICNEOLAIOCHTA BHAILE ATHA CLIATH · DUBLINCoordinatorIreland
Links
Data: CORDIS, © European Union