X-probe · Advanced XFEL and Synchrotron based Probes of Protein Structure and Dynamics

Advanced XFEL and Synchrotron based Probes of Protein Structure and Dynamics

In the cross-disciplinary X-Probe project we have built upon the opportunities created by current X-ray sources and of the new ones recently made operative in Europe and worldwide. We have timely trained a new generation of scientists for the present generation of advanced X-ray facilities. Scientifically, X-probe has impacted on our understanding of protein conformational dynamics from femtoseconds to seconds, one of the most challenging problems in structural biology. We have unveiled the structural dynamics of protein that constitute relevant targets in pharmacology and nanotechnology. X-Probe has advanced the technological field of serial approaches at synchrotron radiation facilities by developing software and hardware solutions that extend the revolutionary possibilities created by X-ray Free Electron Lasers, which combine ultrafast X-ray pulses with high brilliance focusing capabilities. Exploitation of these changing technical capabilities required interdisciplinary collaboration between structural biologists, physical chemists, beamline engineers, software developers and industrial partners. Rapid changes in the state-of-the-art demanded young researchers to be trained to meet new experimental, technical and analysis challenges at the forefronts of structural biology. X-probe addressed this challenge through an interdisciplinary and intersectorial training network incorporating protein micro-crystallisation, sample manipulation, beamline development, micro-focus diffraction, software development, time-resolved diffraction, time-resolved wide angle X-ray scattering, and serial femtosecond crystallography. On-site scientific training and seamless industrial participation was complemented with a scientific mentoring program and training in areas such as strategic project planning, intellectual property, commercialisation and communication skills. We delivered a young team of motivated researchers trained to develop new tools for extracting new insights in protein structural dynamics from femtoseconds to second that exploit the extreme X-ray brilliance of recently developing X-ray user facilities. The very relevant European investments into the European XFEL and SwissFEL as well as the ongoing upgrade of synchrotron radiation sources demanded training of young scientists to fully exploit the scientific and technological avenues opened by this European and national effort, we developed a synergistic approach with technologically advanced enterprises to allow ESRs to convert knowledge and ideas into products and services for society and their own benefit.

Data: CORDIS, © European Union