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H2020Individual fellowship2016–2018

fetISC · Characterizing drivers of intestinal tissue maturation in vitro and in vivo

Horizon 2020 — Marie Skłodowska-Curie Actions

Duration
2016-02-01 → 2018-05-16
EU contribution
€212,195
Participants
1
Scheme
MSCA-IF

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Results in brief

Characterizing drivers of intestinal tissue maturation in vitro and in vivo

Inflammatory bowel disease (IBD) affects millions of European citizens. Currently patients are treated with immunosuppressive palliative therapies and in some cases large segments of patients intestines are surgically resected. There is, however no medical cure. We proposed that IBD patients could benefit from a from epithelial cell transplantations if a suitable source can be identified. Human induced pluripotent stem cells (hiPSC) constitute a promising source of cells for transplantation, but current protocols for directed in vitro differentiation in a defined media produce cells with foetal characteristics. Moreover our group and others (Klein OD lab) recently publish that either during colitis regeneration or during parasitic helminths infections, intestinal cells are reprogrammed into a fetal-like state. In order to develop intestinal cell transplantations as well as in order to understand the cellular and molecular characteristics of IBD it becomes instrumental to understand: how intestinal stem cells are originated during foetal development, characterise their unique molecular properties, and elucidate the mechanisms driving foetal intestinal maturation towards an adult intestinal epithelium.

Data: CORDIS, © European Union

Project objective

One of the challenges in regenerative medicine is to generate adult intestinal stem cells (ISCs) from human induced pluripotent stem cells (hiPSC) in vitro in defined conditions. This will be important in order to establish intestinal cell transplantation therapies and a platform for neonatal disease modelling. These issues constitute the main goal of the proposal. The current protocols to direct intestinal differentiation from hiPSC impede their suitability for human transplantation purposes either because they require mice as tissue culture incubators (teratoma formation), long exposure to calf serum, or generate cells with fetal properties. The immature fetal intestine is distinct from its mature counterpart most notably by its absence of differentiated secretory lineages. Moreover the precise location and developmental stage from which ISCs are specified in fetal intestine as well as the mechanisms that govern the progression towards an adult epithelium remain to be elucidated. In order to generate adult ISCs from pluripotent sources it is instrumental to decipher the molecular mechanisms driving ISC maturation under physiological conditions. The project will be initiated with the fine mapping of ISC origin in the fetal epithelium using cell tracing techniques. Then I will characterize how fetal ISCs acquire adult properties at the molecular level. In the host laboratory we hypothesize that differentially expressed transcription factors are responsible for the unique characteristics of fetal and adult ISCs. Recently, using gene expression profiling comparing fetal and adult intestinal cells I have identified specific endodermal transcription factors overexpressed in the immature structures. I expect through the modulation of such factors to trigger the intestinal maturation. To ensure the transferability of the results into clinics I will combine studies with murine models and cells from human fetal and adult intestines.

Original text from CORDIS.

Participants

  • KOBENHAVNS UNIVERSITET · KOBENHAVNCoordinatorDenmark

Links

Data: CORDIS, © European Union