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H2020Individual fellowship2015–2017

[e-POM-Bioanal] · Electrobioanalytical Applications of Polyoxometalates

Horizon 2020 — Marie Skłodowska-Curie Actions

Duration
2015-06-01 → 2017-05-31
EU contribution
€185,076
Participants
1
Scheme
MSCA-IF-EF-ST

Lines connect the coordinator with its partners.

Results in brief

Electrobioanalytical Applications of Polyoxometalates

In this project we are developing a novel diagnostic tool for easy and accurate detection of disease causing genes by employing electrochemical methods. To develop this tool we have to identify four different types of redox active compounds with distinct redox potential between –0.8V and 0.8V. In addition to this, the redox labels should be compatible with DNA-polymerases, and stable under physiological and PCR working condition. The other important issue is the development of robust,thermostable surface chemistry for DNA grafting, which can withstand the elevated temperature used during enzymatic incorporation. If the above-mentioned critical steps are achieved, the work remaining will be the detection of selected genetic diseases using our electrochemical diagnostic tool and validate the tool with real samples. The diagnostic devise will allow for highly cost-effective, rapid and user-friendly platforms for the screening of known and unknown genetic mutations and known single nucleotide polymorphisms.

Data: CORDIS, © European Union

Project objective

The proposed work will exploit the expertise and know-how of the host institute and the fellow to develop a strategy for the detection and screening of genetic mutations in a highly parallelised fashion.The project has an overall objective to develop an electrochemical single nucleotide polymorphism (SNP) detection strategy by using polyoxometalate (POM) labeled DNA (including PCR primers, dNTPs and ddNTPs) for use in solid-phase melting curve analysis and sold-phase array based primer extension and solid phase ligase mediated detection. In order to achieve this, robust surface for anchoring of DNA probes capable of withstanding elevated temperature for enzymatic reactions either in solid phase melting curve analysis or single dideoxynucleotide incorporation will be developed. The proposed strategies will allow for highly cost-effective, rapid and user-friendly platforms for the screening of genetic mutations and single nucleotide polymorphisms.

Original text from CORDIS.

Participants

  • SORBONNE UNIVERSITE · ParisCoordinatorFrance

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Data: CORDIS, © European Union