MCNTMTDC · Metabolic control on NF-κB activity during dendritic cells-mediated immune tolerance to tumours
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2017-09-01 → 2019-12-22
- EU contribution
- €173,076
- Participants
- 1
- Scheme
- MSCA-IF-EF-ST
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Results in brief
Metabolic control on NF-κB activity during dendritic cells-mediated immune tolerance to tumours
The adaptive immune response to tumours is regulated by an intimate balance between tolerogenic and immunogenic responses to tumour associated antigens. Dendritic cells are key regulators of both protective immune responses and peripheral tolerance. A minor population of DCs, conventional DCs type 1 (cDC1s), is recognized within tumour micro-environment as a key player in the control of cancer by adaptive immunity. They are central inducers of tumour-associated cytotoxic T cell response through their ability to activated them in tumour microenvironment. Although their role in the control of cancer by adaptive immunity is well documented now, the signalling pathways that govern their tumour-related functions are poorly understood. NF-κB signalling has been reported to play a prominent role in DC activation under proinflammatory conditions and also suggested to be required for homeostatic maturation of dendritic cells to maintain peripheral tolerance. This project aims at deciphering the role of NF-κB signalling in cDC1 to fight against cancer. Our findings revealed that NF-κB in cDC1s majorly impacts anti-tumour immunity through excluding the recruitment of cytotoxic T cells to tumour sites. Moreover, our study unravel another signalling pathway controlled by NF-κB in tunour-associated cDC1s than control the tumour growth through the recruitment of cytotoxic T cells. These findings constitute a breakthrough in onco-immunology field because they answer to important questions that could help cancer patients to better respond to immunotherapies.
Data: CORDIS, © European Union
Project objective
BACKGROUND: Dendritic cells (DC) are professional antigen presenting cells able to provide protective immunity to cancer. DC undergo functional maturation that can lead to tolerogenic phenotype associated with immune-suppression in cancer by a still unknown mechanism. Recently the host laboratory revealed a new role of IKKβ/NF-kB signalling in the tolergenic function of DC. However, the upstream regulators of this signalling and its implication in cancer have not yet been addressed. Since malignant cells consume high amounts of nutrients and DC become tolerogenic in low nutrient conditions possibly under the control of the master energy sensor AMP kinase (AMPK), a pseudo-starvation state in tumour-associated DC (TADC) may be responsible of their tolerogenic activity. AIMS: To determine the metabolic role of AMPK and the potential links with IKKb/NF-kB signalling in the tolerogenic activity of TADC in melanoma context. METHODS: I will use mice with a deletion of IKKβ in DC (IkbkbΔItgax) generated in the host laboratory and will generate mice with a deletion of AMPK in DC (Ampka1ΔItgax). Isolated DC will be cultured in high/low nutrient conditions or in the presence/absence of melanoma cells. Activation of AMPK and IKKβ/NF-kB signalling will be analyzed in these conditions and correlated with DC maturation. TADC will be analyzed in vivo using a mouse melanoma model with conditional melanocyte-specific expression of BRafV600E combined with PTEN deletion. The contribution of IKKβ and AMPK activation in TADC during tumour development will be assessed using (i) a skin graft model from BRafV600E mutant mice to IkbkbΔItgax and Ampka1ΔItgax mice and (ii) chimeric mice generated with bone marrow cells from IkbkbΔItgax and IkbkbΔItgax mice transferred to irradiated BRafV600E mutant mice. EXPECTED RESULTS/IMPACT: To understand the tolerogenic activity of TADC, which will give insight into potential strategies for overcoming tumour-induced tolerance in DC vaccination approaches.
Original text from CORDIS.
Participants
- CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisCoordinatorFrance
Links
Data: CORDIS, © European Union