ChemoSense · Elucidating the Mechanisms of Insect’s Chemical Taste to Understand Specific Host-Plant Selection
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2016-12-01 → 2019-01-28
- EU contribution
- €171,461
- Participants
- 1
- Scheme
- MSCA-IF
Lines connect the coordinator with its partners.
Results in brief
Elucidating the Mechanisms of Insect’s Chemical Taste to Understand Specific Host-Plant Selection
Similar to humans and other animals, the sense of taste enables an insect to decide what it eats and drinks. One essential checkpoint for evaluating a plant as suitable food source are gustatory receptors (GRs) and their associated neurons. In concert, they decode the plant’s chemical composition by identifying different nonvolatile compounds that contribute to the insect’s behavioural distinction between edible and toxic. Despite their essential role in the insect’s overall food intake and survival, little is known about GRs and its neurons, especially in plant-feeding beetles. This is surprising given their importance as agricultural and forestry pests, their global distribution and huge species numbers. The EU-funded project ChemoSense, led by the Marie Skłodowska-Curie fellow Dr Stefan Pentzold, aimed at identifying and characterising those gustatory mechanisms that mediate host plant selection in a herbivorous pest, the poplar leaf beetle (Chrysomela populi). These beetles specifically feed on leaves of poplar trees and damage short-rotation plantations worldwide. By combining various state-of-the-art methods, ChemoSense has revealed novel insights in understanding taste sensation and host plant selection at different levels. The beetle’s molecular equipment of GR genes was identified and their spatiotemporal expression was exemplary visualised in different taste organs. Using electrophysiological, microscopic, chemo-analytical and behavioural assays, ChemoSense has also shown how C. populi taste their favourite food plant and how single compounds such as sugars and salicinoids affect the beetles’ feeding choice. The results may pave the way for developing sustainable pest management strategies, e.g. taste-based feeding traps with natural tastants.
Data: CORDIS, © European Union
Project objective
The sense of taste enables an animal to decide what it eats and drinks. Insects are able to discriminate among soluble chemicals via their gustatory receptors (GRs) in order to accept or reject a certain food plant or initiate mating and oviposition. Despite their essential role in the insect’s overall food intake, survival and reproductive success, relatively little is known about insect GRs, especially in beetles. This is surprising given their importance as agricultural and forestry pests, their global distribution and huge species number as herbivorous insects. I aim to identify and characterise GRs that mediate host plant selection in a specialist poplar leaf beetle, Chrysomela populi. These beetles feed on economically important poplar trees and damage timber plantations worldwide. It is hypothesised that the phenolic glucoside salicin in poplar is a major taste stimulant for C. populi and enhances feeding activity. However, it is unknown how salicin or, alternatively, other taste stimulants are detected by, and interact with specific GRs in C. populi to elicit feeding behaviour. I will elucidate these mechanisms by (i) identification of candidate genes encoding GRs in C. populi via tissue specific transcriptome sequencing and transcript visualisation via RNA-FISH, (ii) knock-down of single GR genes via RNAi to test the feeding behaviour of RNAi-silenced beetles, and (iii) test whether any of the taste stimulants from poplar function as in vivo and in vitro ligand for specific C. populi GRs by tissue-specific electrophysiological recordings and heterologous expression, respectively. These results will shed new light on the question how GRs function as regulators for herbivorous beetles to select and consume specific plants, which will significantly advance our knowledge in understanding host plant selection. As poplar leaf beetles are important pests, the results may also pave the way for timely development of sustainable pest management in forestry.
Original text from CORDIS.
Participants
- MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV · MUNCHENCoordinatorGermany
Links
Data: CORDIS, © European Union