ChondUb · Identification and characterisation of novel WWP2 substrates and their role in chondrogenesis and osteoarthritis
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2016-07-01 → 2018-06-30
- EU contribution
- €171,793
- Participants
- 2
- Scheme
- MSCA-IF
Lines connect the coordinator with its partners.
Results in brief
Identification and characterisation of novel WWP2 substrates and their role in chondrogenesis and osteoarthritis
Osteoarthritis (OA), which is characterised by progressive cartilage degradation, is the most common form of arthritis affecting more than 10% of the EU population over 60 years of age. The cause of OA is still not known and there is no cure available. The current treatment involves pain relief and joint replacement surgery. The age of the population in the EU and worldwide increases continuously, therefore understanding of the molecular processes involved in the pathogenesis and progression of OA is of great importance. A network of proteins controlled by regulatory proteins is responsible for the proper functioning of living organisms. Ubiquitination is a process where a small protein is attached to other proteins and causes their breakdown or changes their way of acting. Some proteins are known to regulate cartilage production and degradation. WWP2 is a protein which ubiquitinates other proteins, and is known to regulate production of cartilage (chondrogenesis). We aimed to identify and characterise WWP2 interactors and substrates using a combination of proteomics and molecular biology techniques. In addition, we will investigate the role of WWP2 and its substrates in cartilage biology with the goal to uncover molecular targets for OA treatment and other WWP2-related diseases.
Data: CORDIS, © European Union
Project objective
Osteoarthritis (OA), characterised by cartilage degradation, is the most common form of arthritis affecting >10% of the EU population over 60 years of age. The pathogenesis is only poorly understood. There is no cure available and current treatment involves pain relief and joint replacement surgery. WWP2 is an ubiquitin ligase involved in stem cell differentiation, cancer development and immune responses, and importantly plays crucial roles in chondrogenesis and craniofacial development. However, its role in cartilage biology and OA development has not been elucidated. Ubiquitination regulates most signalling pathways and cellular processes. Previously, I have shown that ubiquitination plays a role in OA development. Here I aim to identify and characterise WWP2 substrates using a combination of novel techniques. In addition, I will investigate the role of WWP2 and its substrates in cartilage biology with the goal to uncover molecular targets for OA treatment and other WWP2-related diseases. The project is aligned with the “Health, Demographic Change and Well-Being” and “Understanding disease” challenges of Horizon2020.The work will be performed under supervision of Dr Weissman, Chief of the Laboratory of Protein Dynamics and Signaling at the National Cancer Institute (US) and at “EULAR Centre of Excellence” at Newcastle University (UK) under supervision of Prof Young. Dr Weissman has an excellent track record in ubiquitin ligases biology and development of drugs targeting them and Prof Young in cartilage biology and mouse models of OA. Therefore, both groups are highly complementary for this project and envisage future collaborations arising from this fellowship.During this multidisciplinary project I will acquire new knowledge and will reinforce my research skills by learning novel techniques. It is a great opportunity to conduct a creative and independent research facilitating achievement of my main career goal to become an independent research group leader.
Original text from CORDIS.
Participants
- UNIVERSITY OF NEWCASTLE UPON TYNE · Newcastle Upon TyneCoordinatorUnited Kingdom
- United States Department of Health and Human Services · Washington D.C.United States
Links
Data: CORDIS, © European Union