STARNANO · Spheroids as a Tool to Assess Realistic long term effects of mixtures of nanomaterials and chemicals
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2018-02-01 → 2020-01-31
- EU contribution
- €158,122
- Participants
- 1
- Scheme
- MSCA-IF-EF-ST
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Results in brief
Spheroids as a Tool to Assess Realistic long term effects of mixtures of nanomaterials and chemicals
Among the many nanomaterials (NMs), graphene-related materials (GRM) have become a subject of intensive scientific and industrial interest. The exceptional mechanical, electrical, thermal and optical properties of these materials make them tremendously attractive for a multitude of different applications. To take advantage of these promising materials without hindering their innovation processes, specific safety issues for human health and the environment have to be addressed. GRM are inevitably released into the environment where aquatic systems tend to be their ultimate sink. Because of their particular physico-chemical properties, GRM are likely to interact with existing environmental contaminants. This may lead to remarkable changes in their fate and therefore bioavailability and impact on biota. Adverse effects of some NMs on aquatic organisms, in particular fish, have already been reported. Although conventional in vivo tests have been routinely used to assess the potential risks of NMs to fish, European regulatory bodies encourage and favor the use of alternative methods for obtaining this kind of data. Isolated rainbow trout hepatocytes maintained in 3D cellular structure mimic the tissue/organ environment, thus represent a more powerful tool to investigate the in vivo effects of NMs. The main objective of the present project was to assess the combined effects of NMs and environmentally relevant pre-existing contaminants. To achieve this goal, two specific objectives were delineated: Specific Objective 1. To determine the mechanisms of toxic action of individual NMs or chemicals Specific Objective 2. Determination of the effect of co-exposure of one NM and an organic substance on their cytotoxicity
Data: CORDIS, © European Union
Project objective
Humans and the environment are simultaneously exposed to a plethora of pollutants. Nanomaterials (NMs) constitute a new class of substances with very particular physico-chemical properties whose use is steadily increasing a wide variety of products. Significant advances have been made in the field of NM toxicology, however most studies fail to consider the influence of NMs on the effects of other substances that co-exist in the environment and in particular the long term effects of these mixtures. In vitro approaches, particularly those allowing long term exposures as three dimensional (3D) cell cultures can generate essential information on the mechanisms underlying the toxicity of very low, environmentally realistic, concentrations of substances that only cause effects after long periods of exposure. The central objective of this proposal is to study the combined effects of NMs and pre-existing chemicals widely distributed in the environment using 3D cell cultures and long-term exposures to perform appropriate assessment of the effects associated with nanotechnology products. Achieving this outcome, the present project will provide an insight into the intracellular fate of NM, the transfer of NMs from cell to cell, and the effects of the combination of NM-organic substances. This information will support the safe and sustainable use of NMs as carriers of other substances (e.g. pesticides or antibiotics) in agriculture or livestock farming. This project addresses some important areas on the ecotoxicity of NMs declared as needed within the framework of REACH regulation. The topic of this project is directly related to the Social Challenges (agriculture, environment and health) and Industrial Leadership (nanotechnology) strategic policy objectives of Horizon 2020 programme. Moreover, the use of this experimental approach is requested under the call for “Science-based risk assessment and management of nanotechnologies” of the above mentioned work programme.
Original text from CORDIS.
Participants
- AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS · MadridCoordinatorSpain
Links
Data: CORDIS, © European Union