FASTR 3D SIM · Focus on Advancing Spatial and Temporal Resolution of 3D Structured Illumination Microscopy
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2017-11-06 → 2019-11-05
- EU contribution
- €160,800
- Participants
- 1
- Scheme
- MSCA-IF-EF-ST
Lines connect the coordinator with its partners.
Results in brief
Focus on Advancing Spatial and Temporal Resolution of 3D Structured Illumination Microscopy
Fluorescence microscopy is an important tool in biological and biomedical research, as it allows researches to visualize structures and dynamics on a cellular and sub-cellular level. Two common limitations in the study of inner- and intra-cellular dynamics are the resolution and the speed that can be achieved. Light diffraction limits how fine the structures are that a microscope can resolve, and technical implementation limits how fast dynamics can be observed. This project aimed to improve both the speed and the resolution with which we can observe those dynamics. More specifically, new technical solutions (implementing faster microscopes based on novel optical elements) and the improvement and combination of existing microscope methods, are used to achieve this goal. The successful implementation will provide vital tools for biological and biomedical research, especially those fields aiming to observe fast dynamics at highest resolutions. For example, the visualization of mitochondrial dynamics require both the resolution and speed we aim to provide.
Data: CORDIS, © European Union
Project objective
FASTR 3D SIM: Focus on Advancing the Spatial and Temporal Resolution of 3D Structured Illumination MicroscopySuper-resolved structured illumination microscopy (SR-SIM) is an important method to surpass the resolution limit in fluorescence microscopy. SR-SIM is compatible with many fluorescent labels and ensures low photo-damage, making it an ideal candidate for live-cell imaging. However, to be truly applicable, very fast 3D imaging has to be realized, as the cellular structuring is 3D in nature and fast dynamics have to be captured. In addition, many biological processes occur at length scales below 100nm, beyond SR-SIMs two-fold resolution improvement.I will develop an advanced 3D SR-SIM microscope able to capture 3D images at video rate, i.e., 25 3D images per second, 10 times quicker than the fastest currently available systems. The extreme speed-up is achieved by a unique multi-focal optics approach available at the host institute, and will also reduce the photo-toxicity to the sample. My SIM reconstruction code (www.fairsim.org) will be extended to 3D imaging, and include new algorithms tackling low light levels and strong out-of-focus light.I will also develop a revolutionary combination of SIM with super-resolution optical fluctuation imaging (SOFI), a specialty of the Dedecker lab. SOFI is employed to create non-linear effects, allowing SIM to surpass its 2x resolution enhancement. This enables rapid and repeatable 3D imaging of cells with a spatial resolution approx. 60 nm in x,y and 150 nm in z.I will apply the tools to an acute biological question, the dynamics of mitochondrial and ER interactions, used by the cell as signaling scaffolding points. Here both the temporal and spatial resolution of the proposed system is required to observe the very fast dynamics.
Original text from CORDIS.
Participants
- KATHOLIEKE UNIVERSITEIT LEUVEN · LeuvenCoordinatorBelgium
Links
Data: CORDIS, © European Union