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H2020Staff exchange2018–2023

CLATHROPROBES · Chiroptical, optical and magnetic probes for protein sensing based on cage metal complexes

Horizon 2020 — Marie Skłodowska-Curie Actions

Duration
2018-01-01 → 2023-12-31
EU contribution
€796,500
Participants
8
Scheme
MSCA-RISE

Lines connect the coordinator with its partners. CORDIS does not always give exact coordinates for projects before 2014. These points are placed at city or country level.

Results in brief

Chiroptical, optical and magnetic probes for protein sensing based on cage metal complexes

The CLATHROPROBES project has been devoted to the design of novel and highly efficient chiroptical, luminescent, and other probes for sensing of proteins and their structural changes, build on the basis of cage metal complexes (clathrochelates and lacunar spatial complexes with encapsulated 3d metal ions) as molecular reporters. The majority of known probes for protein sensing or inhibitors of protein activity is based on organic compounds built on a carbon-based scaffold. The highest number of substituents on a carbon atom is 4. In contrast, metal-based scaffolds offer higher number of substituents that significantly expand the available structural space and allow efficient targeting of a variety of proteins. However, this potentially powerful approach has been rarely used up to now, which is arguably caused by challenges in synthesis of metal-based protein binders stable in the presence of natural metal binders like amino acids, peptides, metallothioneins or others. The aim of CLATHROPROBES has been to develop metal cage complexes as molecular reporters able to sense target proteins and structural changes that they undergo. Since the “reporting properties” of cage complexes allow direct studies of their interactions with a target, this research opens new opportunities for the design of “smart” cage molecules - imaging probes and therapeutic agents. This project included the design and synthesis of new compounds, their complete structural and spectroscopic characterization, studies of their interactions with proteins, properties as reporters, and evaluation of their stability, bioactivity and cytotoxicity in model systems.

Data: CORDIS, © European Union

Project objective

The CLATHROPROBES project is devoted to the design and development of novel, highly efficient chiroptical, luminescent, IR, NMR and EPR probes for sensing and structural studies of biomolecules based on cage metal complexes (clathrochelates and lacunar complexes of spatial tris-diiminate ligands with encapsulated 3d-metal ion) as molecular reporters.For successful design and synthesis of cage metal complexes (WP1), their complete structural and spectroscopic characterization (WP2), studies of their interactions with biomolecules and reporting properties, and their cytotoxicity and bioactivity in model systems (WP3), the project brings together researchers from four academic institutions and two industrial companies, representing six countries (PL, DE, UA, RO and AU; RU - participation till April 8th, 2022). The participants will contribute to the project with different, highly specific, yet complementary expertise, namely in chemical synthesis of clathrochelate complexes (PBMR and FAU; INEOS RAS participation till April 8th, 2022), identification and structural studies of compounds and materials (PBMR, FAU, UWR, UNIVIE, and SPS; INEOS RAS - participation till April 8th, 2022), characterization of interactions between ligands/clathrochelates/materials and biomolecules by CD, UV-VIS, fluorescence ITC, IR, NMR methodology (UWR, FAU, PBMR, UNIVIE, SPS), and biological studies (FAU).Joint efforts of participants of this multidisciplinary project will provide - the transfer of knowledge between scientists of different research areas, sectors and countries that will enhance level of their professional scientific/technical expertise, -training of young researchers, very important for their further scientific success or industrial career, - enhancement of research activity and network of participating Institutions, - new long-term collaborations between the partners, - increase of academia-industry interactions, so valuable for innovative ideas and discoveries, and pair scientific excellence with social awareness and understanding of current tasks and problems of science.

Original text from CORDIS.

Participants

  • UNIWERSYTET WROCLAWSKI · WroclawCoordinatorPoland
  • A.N. NESMEYANOV INSTITUTE OF ORGANOELEMENT COMPOUNDS OF RUSSIAN ACADEMY OF SCIENCES · MOSKVARussia
  • FRIEDRICH-ALEXANDER-UNIVERSITAET ERLANGEN-NUERNBERG · ErlangenGermany
  • INNOVATION DEVELOPMENT CENTER ABN LLC · KIEVUkraine
  • SARA PHARM SOLUTIONS SRL · BucurestiCity levelRomania
  • SRL POLIVALENT-95 · CHISINAUMoldova
  • SUBSIDIARY COMPANY PRINCETON BIOMOLECULAR RESEARCH LABS · KIEVUkraine
  • UNIVERSITAT WIEN · WienAustria

Links

Data: CORDIS, © European Union