SUPRAforORGANS · Synthetic supramolecular polymers as artificial extracellular matrix for stem cell expansion towards organoids.
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2019-02-01 → 2021-01-31
- EU contribution
- €177,599
- Participants
- 1
- Scheme
- MSCA-IF
Lines connect the coordinator with its partners.
Results in brief
Synthetic supramolecular polymers as artificial extracellular matrix for stem cell expansion towards organoids.
One of the most urgent challenges in medicine is to devise new strategies for replacing organs. The number of organ replacements not only exceeds by far the supply of organs available for transplantation but is also likely to increase dramatically. Stem cells are a promising tool due to their capacity to proliferate and to differentiate into mature cells of various types; therefore, in principle stem cells could be used to replace any organ in the body. The fate of stem cells is governed by the microenvironment (or niche) they foster, which offers the main point of control. Unfortunately, the lack of an optimum extracellular matrix (ECM) that closely mimics the in vivo microenvironments to promote differentiation of stem cells is the main limitation of this technology. On this basis, the objective of this project is to develop an artificial ECM based on a hydrogel form by synthetic supramolecular polymers to achieve a system that can support the growth of stem cells into organoids. Importantly, supramolecular polymers are dynamic since their monomers are bound by non-covalent interactions. This property will allow cells to actively adapted the hydrogel, inducing both depolymerisation and reassembly to continuously support the growing organoids. In summary, the project has achieved to develop a strategy to prepare BTA hydrogels with tunable stiffness, and also found a new technology to bind chemical functionalities to BTA supramolecular polymer. In the future, the performance of these approaches to induce stem cell differentiation need to be evaluated.
Data: CORDIS, © European Union
Project objective
One of the most urgent challenges in medicine is to devise new strategies for replacing organs. The most promising approach to tackle this limitation is the use of stem cells. These cells have the capacity to proliferate and to differentiate into mature cells of various types. In fact, embryonic stem cells can become any organ, therefore, in principle, stem cells could be used to replace any part of the body. The fate of stem cells is governed by the microenvironment they foster, which offers the main point of control. Unfortunately, the lack of an optimum extracellular matrix that closely mimics the in vivo microenviroments to promote differentiation of stem cells is the main limitation of this technology.The SUPRAforORGANS uses a radically different strategy for designing an ideal artificial extracellular matrix. This approach consists of applying a dual network hydrogel formed by two different synthetic supramolecular polymers. One of the polymers will form a stiff and fairly stable fibre that takes care of optimize the mechanical properties of the gel, and will have binding cell surface receptors required for cell expansion. The second phase will be a weaker dynamic component in which biological cues are intercalated to bind and stabilize growth factors and other nutrients necessary for the cells to grow and organize into organoids. This matrix can actively be adapted by the cell, inducing both depolymerisation and reassembly to continuously support the growing organoids. Furthermore, some of cell receptors will be protected with photo-labile groups to possess user-defined spatio-temporal control, thereby this could control the three-dimensional growth of the organoid.
Original text from CORDIS.
Participants
- TECHNISCHE UNIVERSITEIT EINDHOVEN · EindhovenCoordinatorNetherlands
Links
Data: CORDIS, © European Union