HI-PHRET · High-resolution Imaging with Phase Retrieved Tomography
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2019-03-01 → 2021-02-28
- EU contribution
- €180,277
- Participants
- 1
- Scheme
- MSCA-IF
Lines connect the coordinator with its partners.
Results in brief
High-resolution Imaging with Phase Retrieved Tomography
Optical tomography, the ability to inspect biological organisms without affecting their functional and vital structures, is an evolving research area that encourages advancement in the study of biological processes. However, modern measurement methodologies sometimes fail to meet the demands of biomedical imaging. In particular, the process of light diffusion through opaque tissues and problems due to alignments of the experimental setup enormously limit the quality of the results obtainable with conventional microscopy techniques. In this context, the idea behind the "High-resolution Imaging with Phase Retrieved Tomography" project (HI-PHRET) is to reach the maximum tomographic resolution assisted by an effective imaging pipeline. To do this, we put cutting-edge photonics and computational phase retrieval techniques at the service of the biomedical imaging world. Mechanical misalignments, light scattering problems, and imaging of objects hidden behind opaque structures were tackled with new implementations of multi-dimensional phase retrieval methods. The project has been structured in three phases, interfacing theory, experiments, and using tailored computational methods: - We developed a new multi-dimensional and multispectral phase retrieval techniques for the inversion of autocorrelation, by exploiting the computational power offered by GPUs. - We exploited these methods for the tomographic reconstruction of the fluorescent distribution from biological samples in an optical microscopy setup. Our technique is free from any alignment issues, permits the reconstruction accurate at the sub-pixel level, and achieved a resolution higher than the state-of-art methods. - Further, we extended the method to perform hidden tomography without the use of lenses, reconstructing samples not directly visible due to the presence of an optically opaque barrier.
Data: CORDIS, © European Union
Project objective
Optical tomography is a modern field of indisputable interest that fosters advances in the study of biological processes. Even if this scientific pairing seems to proceed tightly bind, sometimes current methodologies vacillate to satisfy imaging requests coming from the medical world. In this scenario, the intention of High-resolution Imaging with Phase Retrieved Tomography (HI-PHRET) project, is to achieve the maximum tomographic resolution taking advantage of cutting-edge photonics and computational techniques. In fact, Phase Retrieval (PR) methods are unveiling their promising imaging capabilities since, with the current technology, we are able to measure a “mere” amplitude-only image. The recovery of phase information opens up numerous possibilities, drafting the goal of the project: put PR algorithms at the service of the biomedical imaging world. Mechanical misalignment, scattering and hidden imaging can be efficiently handled with PR implementations, further advancing towards the highest tomographic resolution. HI-PHRET will thrive at Politecnico di Milano, under the supervision of Prof. Pifferi, and will project me into the excellences of the European scientific panorama. Thanks to the possibilities offered by the prestigious MSCA-IF and by the inclusion in a well-structured multi-disciplinary network, I will be able to grow professionally maturing further independent-thinking and project-management skills. The interaction with universities, research institutes, industries and companies (also by means of two secondments), as well as direct contact with technology-transfer offices, will open up new possibilities for my career and maximise the impact of the research action. Dissemination, exploitation and communication strategies planned throughout the project will encourage me to think different, trying to catch the attentions of the broadest audience but focusing on concrete problematics, always keeping an eye on potential technology transfer.
Original text from CORDIS.
Participants
- POLITECNICO DI MILANO · MilanoCoordinatorItaly
Links
Data: CORDIS, © European Union