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H2020Doctoral network2019–2023

ENDONANO · Quantitative detection of bacterial endotoxin by novel nanotechnological approaches

Horizon 2020 — Marie Skłodowska-Curie Actions

Duration
2019-01-01 → 2023-06-30
EU contribution
€1,035,724
Participants
9
Scheme
MSCA-ITN

Lines connect the coordinator with its partners.

Results in brief

Quantitative detection of bacterial endotoxin by novel nanotechnological approaches

ENDONANO addresses a key regulatory and safety issue, i.e., the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances, especially for nanomedicine-based formulations. ENDONANO will exploit new concepts and knowledge, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells for new cell-based LPS detection assay; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes.

Data: CORDIS, © European Union

Project objective

ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory and safety issue, i.e., the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs). All have proven experience in higher education and training,

Original text from CORDIS.

Participants

  • CONSIGLIO NAZIONALE DELLE RICERCHE · RomaCoordinatorItaly
  • ALTA RICERCA E SVILUPPO IN BIOTECNOLOGIE SRLU · SienaItaly
  • APPLIED NANOPARTICLES SL · BarcelonaSpain
  • BLUSENSE DIAGNOSTICS APS · KobenhavnDenmark
  • DIAGNOSTICUM GYARTO ES FORGALMAZO ZARTKORUEN MUKODO RESZVENYTARSASAG · BudapestHungary
  • FUNDACIO INSTITUT CATALA DE NANOCIENCIA I NANOTECNOLOGIA · BELLATERRA (BARCELONA)Spain
  • MILTENYI BIOTEC BV & CO KG · Bergisch GladbachGermany
  • MILTENYI BIOTEC GMBH · Bergish GladbachGermany
  • PARIS-LODRON-UNIVERSITAT SALZBURG · SalzburgAustria

Links

Data: CORDIS, © European Union