MesoBrainMicr · Novel high speed and high resolution microscopy setup for cytoarchitectonic studies of mesoscale sized human brain tissues, healthy and affected by Focal Cortical Dysplasia
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2018-10-01 → 2020-09-30
- EU contribution
- €180,277
- Participants
- 1
- Scheme
- MSCA-IF-EF-ST
Lines connect the coordinator with its partners.
Results in brief
Novel high speed and high resolution microscopy setup for cytoarchitectonic studies of mesoscale sized human brain tissues, healthy and affected by Focal Cortical Dysplasia
The human brain is a complex information processing system with a hierarchy of different yet tightly integrated levels of organization. From such intricate interplay emerge our personality, emotions, memory and decision making capabilities, but understanding its functioning is a huge challenge. An open problem is how to investigate with both high resolution and high speed the brain structure over mesoscale (millimeters to centimeters) sized regions, which would provide unique insight on its neuroanatomy and help to develop novel medical diagnostic approaches. In this project we aim to develop a one photon fluorescence microscope capable of resolving sub-cellular morphology over centimeter-sized tissue samples at state of the art speed and with micrometric resolution. This innovative instrument, called dual-view inverted dual-slit confocal light sheet fluorescence microscope (di2CLSFM), will enable new studies of the brain anatomy across different functional areas. One traditional limitation of cytoarchitecture studies is their limited extension. Cell morphology and spatial distribution are analysed within single cortical columns or small brain volumes, potentially hiding any long-range correlations in the fine details of neuronal organization or missing unexpected neuroanatomical features in other brain areas. We will use the di2CLSFM to obtain a detailed characterization of the human brain cytoarchitecture, both on the level of local circuits and of long-range connections, in healthy and dysplastic mesoscopic tissues, in particular affected by Focal Cortical Dysplasia (FCD). This pathology is a common malformation of cortical development found in epilepsy surgeries and its causes are poorly understood. This approach will generate digital anatomical reconstructions of large brain volumes which will greatly advance the medical and neurobiological understanding of the healthy brain tissue structure and of the effects of neuro-degenerative pathologies.
Data: CORDIS, © European Union
Project objective
The human brain is a massively complex information processing system with a hierarchy of different yet tightly integrated levels of organization. From such intricate interplay emerge our personality, emotions, memory and decision making capabilities, but unwrapping the mysteries of the brain functioning represents a huge challenge.Recent progress in microscopy, neurobiology and neuroinformatics has stimulated the launch of large research programs such as Human Brain Project and BRAIN. An open problem is how to investigate with both high resolution and high speed the brain structure over mesoscale (millimiters to centimeters) sized regions, which would provide unique insight on the neuroanatonomy across different functional areas. This research project will develop a novel dual-view inverted dual-slit confocal light sheet microscope, capable of resolving sub-cellular morphology over centimeter-sized tissues at beyond state of the art acquisition speed. This instrument will be used to perform a comparative cytoarchitectonic investigation of mesoscale human brain tissues, both healthy and affected by Focal Cortical Dysplasia, leveraging an advanced machine learning image analysis algorithm. Such study will greatly advance the medical and neurobiological understanding of the effects of neuro-degenerative pathologies and the obtained data will be contributed to existing human brain atlases as a foundation for brain models.The applicant’s well-rounded skill set, acquired in cold atom physics and ranging from optics to hardware control, is a perfect match for building the proposed setup and for a fruitful two-way transfer of knowledge with the host, expert in advanced neuro microscopy.This research project will strengthen the competitiveness of European science and shine new light on the human brain anatomy, consequently it will raise the applicant's international recognition, and establish him inside Europe as a leading researcher in this quickly evolving field.
Original text from CORDIS.
Participants
- UNIVERSITA DEGLI STUDI DI FIRENZE · FlorenceCoordinatorItaly
Links
Data: CORDIS, © European Union