HAZARDOmics · Liquid Chromatography-Ion Mobility-Mass Spectrometry and Capillary Electrophoresis-Mass Spectrometry as ground-breaking approaches to expand the boundaries of metabolomics in chemical risk assessment
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2018-10-01 → 2021-03-31
- EU contribution
- €185,076
- Participants
- 1
- Scheme
- MSCA-IF-EF-ST
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Results in brief
Liquid Chromatography-Ion Mobility-Mass Spectrometry and Capillary Electrophoresis-Mass Spectrometry as ground-breaking approaches to expand the boundaries of metabolomics in chemical risk assessment
During our life, we are exposed to various chemical agents such as food contaminants, environmental pollutants, drugs, allergens, etc., some of which are endocrine disrupting-compounds (EDCs) such as bisphenol A or polychlorinated biphenyls (PCBs). Therefore, these chemicals can cause adverse effects on our health and cause chronic diseases. From a public health perspective, it is of great interest to understand the risks associated with exposure to chemicals and the underlying modes of action (MoA) of these chemicals for the effective implementation of health prevention strategies. In this sense, chemical risk assessment is a great challenge for today’s society considering the large number of chemical substances available on the European Union (EU) market. In addition, to implement effective prevention strategies towards chemical exposure, chemical risk assessment is required to address health effects related to current exposure scenarios beyond the unrealistic and high exposure doses traditionally used in toxicological studies. Within this framework, metabolomics has recently emerged as an innovative approach to address the current challenges of chemical risk assessment, including exposure to chemical mixtures and low dose chemical exposures. The HAZARDOmics project applies metabolomics as a novel strategy in risk assessment to generate new knowledge about the effects of chemicals related to exposure to low levels of BPA and PCBs. Furthermore, HAZARDOmics also involves the application of less frequent analytical tools in metabolomics studies, specifically ion mobility spectrometry (IMS) and capillary electrophoresis-mass spectrometry (CE-MS), for risk assessment of BPA and PCBs. Specifically, HAZARDOmics pursues analytical objectives: 1) expanding the current technical boundaries of metabolomics in risk assessment by integrating IMS into traditional liquid-chromatography-high resolution mass spectrometry (LC-HRMS) workflows, 2) implementing CE-HRMS as a complementary analytical technique to support metabolomics in risk assessment; as well as specific risk assessment objectives: 3) the identification of effect biomarkers related to exposure to a PCB ‘cocktail’ within a risk assessment framework, 4) the discovery of effect biomarkers associated with exposure to low doses of BPA.
Data: CORDIS, © European Union
Project objective
Nowadays, there is a growing concern in the research community and civil society in general about the effects of chemical exposure on human health. From a public health perspective, it is of major interest to establish the risks associated with chemicals for the enforcement of health prevention strategies. In this sense, one of the current priorities of chemical risk assessment is to understand the effects and modes of action (MoA) of endocrine disruptor compounds (EDCs) such as bisphenol A (BPA) and polychlorinated bisphenyls (PCBs). Within this framework, metabolomics has emerged as a novel strategy for the discovery of effect biomarkers that can provide the connection between exposures to EDCs and chronic diseases.HAZARDOmics aims to enhance the knowledge about the risks arising from exposures to low doses of BPA and to a ‘cocktail’ of PCBs following an innovative metabolomics approach. The metabolic profiling of blood serum samples will be investigated from a broad analytical perspective in order to obtain the most complete information of metabolic fingerprint and, consequently, to increase the possibility of identifying effect biomarkers. Liquid chromatography (LC)-High Resolution Mass Spectrometry (HRMS) involving Reverse Phase Liquid Chromatography (RP-LC) and Hydrophilic Interaction Liquid Chromatography (HILIC) will be employed as traditional metabolomics strategy in risk assessment. On the other hand, the boundaries of this approach will be extended by the application of two additional techniques. For the first time, Ion Mobility Spectrometry (IM) integrated in a LC-HRMS workflow will be evaluated as a ground-breaking technology for metabolomics intended to risk assessment purposes. Moreover, Capillary Electrophoresis (CE)-HRMS will be implemented as complementary technique to LC-HRMS since both involve different separation mechanisms.
Original text from CORDIS.
Participants
- ECOLE NATIONALE VETERINAIRE, AGROALIMENTAIRE ET DE L'ALIMENTATION NANTES ATLANTIQUE · NantesCoordinatorFrance
Links
Data: CORDIS, © European Union