T-REX · Developing healthy crops for EU: T3SS-Recognition EXploration (T-REX) for plant immunity against bacteria
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2019-01-14 → 2021-01-13
- EU contribution
- €200,195
- Participants
- 1
- Scheme
- MSCA-IF-EF-ST
Lines connect the coordinator with its partners.
Results in brief
Developing healthy crops for EU: T3SS-Recognition EXploration (T-REX) for plant immunity against bacteria
Plants, animals and humans often suffer from bacterial diseases, which seriously affect production and well-being. Pathogenic bacteria use the needle-like type-3 secretion system (T3SS) to inject effector proteins into the cells of the hosts. At the same time, it is important for the hosts to be able to recognize invading pathogens, in order to activate their immune system when attacked. Therefore, it is surprising that it has not previously been demonstrated that plants can recognize the T3SS, as it is indispensable for bacterial pathogens. Meanwhile, this project was based on our finding that many wheat cultivars indeed do recognize the T3SS from a Pseudomonas pathogen, and that the wheat gene responsible for this should be found between seven candidates. The objectives of the project were 1) to identify which of the approx. 25 T3SS proteins is recognized by wheat, 2) to identify which wheat protein functions as receptor to detect this T3SS protein, and 3) to evaluate whether the recognition activates immunity that protects the plant. Understanding the molecular details of this recognition can potentially be exploited to control serious bacterial diseases in plants and animals. Such strategies include use of selected naturally occurring gene variants, molecularly modified genes or drugs inspired by the structures of the interacting molecules.
Data: CORDIS, © European Union
Project objective
Among the greatest challenges of the 21st century are bacterial diseases that permanently threat plants and animals, including humans to whom they often are lethal. In plant and animal production, bacteria can cause severe yield reductions if not effectively controlled. However, the current control measures include use of environmentally problematic antibiotics, which in addition may lead to appearance of bacterial strains resistant to these antibiotics. Recent progress in the host laboratory using a Genome Wide Association Study (GWAS) of wheat reveals a hitherto unknown recognition mechanism of the effector translocator widely conserved in pathogenic bacteria. The associated receptor complex is predicted to have wide potential for exploitation in bacterial disease control, not only in crop plants but also in livestock production. In the T-REX project, number of parallel efforts will be taken to characterize the recognized T3SS component, the membrane bound receptor complex and the activated immunity. This will be undertaken using an integrated approach, which involves my background knowledge and experiences combined with the ones of the host institution within molecular genetics, biochemistry, molecular biology and bioinformatics. This study will shed light on the role of T3SS as a PAMP, which will help us to elucidate the complex plant-bacterial interactions and can potentially lead to the development of new phyto-pathogen control strategies. T3SS being conserved among plant and animal pathogen, the outcome of the T-REX project has potential for extrapolation into animal pathological systems, including humans, and may contribute to the world of medicine.
Original text from CORDIS.
Participants
- KOBENHAVNS UNIVERSITET · KOBENHAVNCoordinatorDenmark
Links
- View on CORDIS
- DOI: 10.3030/795806
- https://plen.ku.dk/english/research/plant_soil/pdg/research-themes/signalingforimmunity/
Data: CORDIS, © European Union