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H2020Individual fellowship2021–2024

VacTrack · Dissecting the protection mechanism of new generation vaccines by synchronizing systems biology with in vivo imaging

Horizon 2020 — Marie Skłodowska-Curie Actions

Duration
2021-12-13 → 2024-12-12
EU contribution
€281,359
Participants
2
Scheme
MSCA-IF

Lines connect the coordinator with its partners.

Results in brief

Dissecting the protection mechanism of new generation vaccines by synchronizing systems biology with in vivo imaging

Despite successful reduction of the burden of many infectious diseases by vaccination and the strong improvement of vaccine safety, there is a strong need to improve existing vaccines and to develop new affordable vaccines with greater efficacy. Particle-based vaccines are one of the recent advances, with potentially a promising impact on global public health. Besides being investigated for their protective potential, the knowledge about their mechanism of protection is largely unknown.. VacTrack was designed to investigate this by innovatively combining state of the art in vivo imaging and high-dimensional flow cytometry.. I aimed to dissect the route vaccines acquire inside the host along with the spatio-temporal changes inimmune rertoire following vaccine exposure to the host, by utilizing pneumococcal antigens grafted to outer membrane vesicles (OMVs) as model formulations in imaging-assisted in vivo mouse vaccination models. By exploiting high resolution in vivo imaging and immunological approaches, VacTrack aimed to provide a blue print for the approach to study mechanism of protection of vaccines in general. Comprehension of correlates of protection and following the kinetics of changes in the immune repertoire of host niches post vaccination aimed to 1) help devise new pneumococcal vaccines, 2) improve existing vaccine formulations, 3) transform the direction of vaccine research by allowing targeting of specific novel pathways identified via VacTrack. Taken together, this project aimed to provide important insights in advancing the vaccine research to devise new and better vaccines and to save and improve human lives on a global level. After publishing the results, the outcomes will be published to the webo=site of host organization. Since the manuscript is still in progress, there in no content published on the website till now.

Data: CORDIS, © European Union

Project objective

Despite successful reduction of the burden of many infectious diseases by vaccination and the strong improvement of vaccine safety, there is a strong need to improve existing vaccines and to develop new affordable vaccines with greater efficacy. Particle-based vaccines are one of the recent advances, with potentially a promising impact on global public health. Besides being investigated for their protective potential, the knowledge about their mechanism of protection is still scarce. VacTrack is designed to investigate this by innovatively combining state of the art in vivo imaging with global transcriptome analyses. I aim to dissect the route vaccines acquire inside the host along with the spatio-temporal changes in gene expression following vaccine exposure to the host, by utilizing pneumococcal antigens grafted to outer membrane vesicles (OMVs) as model formulations in imaging-assisted in vivo mouse vaccination and challenge infection models. VacTrack is expected to generate insights about novel pathways triggered in host following vaccination which will be further validated by an ex vivo approach via stimulation of murine and human whole blood. By exploiting systems biology, high resolution in vivo imaging and immunological approaches VacTrack will provide a blue print for the approach to study mechanism of protection of vaccines in general. Comprehension of correlates of protection by combining in vivo and ex vivo analyses will 1) help devise new pneumococcal vaccines, 2) improve existing vaccine formulations, 3) transform the direction of vaccine research by allowing targeting of specific novel pathways identified via VacTrack. Taken together, this proposal holds great potential in advancing the vaccine research to devise new and better vaccines to save and improve human lives on a global level.

Original text from CORDIS.

Participants

  • STICHTING RADBOUD UNIVERSITAIR MEDISCH CENTRUM · NijmegenCoordinatorNetherlands
  • STICHTING RADBOUD UNIVERSITEIT · NijmegenNetherlands

Links

Data: CORDIS, © European Union