Menu
MapConnectionsTopicsBulgariaThe Night (K-TRIO)Marie Skłodowska-CurieFor studentsFor teachersMethodologySurprise me
H2020Individual fellowship2021–2023

SNiB-cdG-P · Global mapping of second messenger c-di-GMP signaling networks in bacteria using proteomics

Horizon 2020 — Marie Skłodowska-Curie Actions

Duration
2021-03-01 → 2023-02-28
EU contribution
€191,149
Participants
1
Scheme
MSCA-IF

Lines connect the coordinator with its partners.

Results in brief

Global mapping of second messenger c-di-GMP signaling networks in bacteria using proteomics

Bacteria are masters in sensing environmental cues and in processing this information to adequately adjust their behavior. A key element of bacterial signal transduction is the nucleotide second messenger cyclic di-guanosine-monophosphate (c-di-GMP). This powerful signalling molecule acts in minute amounts to control important cellular functions, such as motility, biofilm formation, or bacterial virulence and persistence. Despite of its global importance in regulating bacterial growth and behavior, studies systematically investigating the c-di-GMP network in bacteria are missing. To address this, the project aimed to employ recently developed quantitative proteomics techniques to identify and characterize proteins involved in c-di-GMP signaling pathways in bacterial model organisms.

Data: CORDIS, © European Union

Project objective

To survive in diverse niches, bacteria must adapt to changes in their local environment by sensing and responding to environmental cues. External cues are transduced through complex signaling networks throughout a cell and drive diverse changes in cellular behavior. In bacteria, cyclic di-guanosine-monophosphate (c-di-GMP) is a nucleotide-derived second messenger that mediates signal transduction of important biological processes for bacterial growth and survival e.g. motility, biofilm formation and metabolism. These biological processes are also crucial in clinical settings as they underlay antibiotic resistance in important pathogenic bacteria. Recent advances in MS-based proteomics have provided different tools to investigate the proteome of an organism in a systematic and global manner. Especially, thermal proteome profiling (TPP) and limited-proteolysis MS (LiP-MS) are pioneering methods to study change of protein states proteome-wide. I aim to employ these proteomics based approaches to achieve the global map of the c-di-GMP signaling network in two different bacteria, Escherichia coli and Caulobacter crescentus, both are model organisms of Gram-negative bacteria. Furthermore, I will apply these methods to investigate signaling network of another important second messenger, (p)ppGpp, and explore how the networks of these two messenger molecules interact in bacteria to dictate cellular physiology.

Original text from CORDIS.

Participants

Links

Data: CORDIS, © European Union