POP1Seed · Population-wide analysis of molecular and metabolic features intrinsic to dormancy variability in single seeds
Horizon 2020 — Marie Skłodowska-Curie Actions
- Duration
- 2021-12-01 → 2023-11-30
- EU contribution
- €137,626
- Participants
- 1
- Scheme
- MSCA-IF
Lines connect the coordinator with its partners.
Results in brief
Population-wide analysis of molecular and metabolic features intrinsic to dormancy variability in single seeds
Seed physiological quality is pivotal to ensure rapid and homogeneous seedling establishment required for stable yield and is a major economic challenge for the seed industry. Besides germination, vigor and storability or longevity, seed physiological quality also includes seed dormancy, i.e. the seed physiological stage preventing germination even under favorable conditions. While seed dormancy has been widely studied at population level, little has been done at single seed level. However, homogeneous seed lot production is crucial in plant breeding in the context of climate change. In this project, POP1SEED, we aim to decipher novel regulators of single seed dormancy by GWAS approach. There are two sub-objectives: (i) Characterize single seed variability within A. thaliana accessions at both physiological and metabolic levels and identify new putative genomic regions involved in single seeds dormancy depth by Genome Wide Analysis (GWAS) (ii) Identify whether dormancy variability is cis or trans regulated and to identify the putative candidates at gene level using single seed RNA sequencing
Data: CORDIS, © European Union
Project objective
Seed physiological quality is pivotal to ensure rapid and homogeneous seedling establishment required for stable yield and is a major economic challenge for the seed industry. Besides germination, vigor and storability or longevity, seed physiological quality also includes seed dormancy, i.e. the seed physiological stage preventing germination even under favorable conditions. While seed dormancy has been widely studied at population level, little has been done at single seed level. However, homogeneous seed lot production is crucial in plant breeding. In seeds, dormancy is mainly regulated by DELAY OF GERMINATION 1 (DOG1), a major Quantitative Trait Locus controlling seed dormancy. DOG1 expression is highly regulated, including by alternative polyadenylation which resulted in the discovery of functional DOG1 protein variant. However, whether this major QTL also regulates individual seed dormancy variability is still unknown. Here, this project combines several cutting-edge technologies such as single seed RNA sequencing analysis and mass spectrometry imaging to Genome Wide Analysis and mutant characterization to unravel novel regulators of single seed dormancy in Arabidopsis thaliana, with the perspectives to be applied on rapeseed. While improving my career prospects and learning from the establishment of international collaborations between the host institute in Poland and the academic partner IBMP-CNRS in France, this project POP1Seed is timely, and responds to expectations of the European Framework programme for Research and Innovation.
Original text from CORDIS.
Participants
- INSTYTUT BIOCHEMII I BIOFIZYKI POLSKIEJ AKADEMII NAUK · WarszawaCoordinatorPoland
Links
Data: CORDIS, © European Union