LIPIRNADO · Targeted delivery of lipid nanoparticle-mRNA formulations modified with ligand pattern to dendritic cells based on DNA origami
Horizon Europe — Marie Skłodowska-Curie Actions
- Duration
- 2023-08-01 → 2025-07-31
- EU contribution
- €222,728
- Participants
- 1
- Scheme
- HORIZON-TMA-MSCA-PF-EF
Lines connect the coordinator with its partners.
Results in brief
Targeted delivery of lipid nanoparticle-mRNA formulations modified with ligand pattern to dendritic cells based on DNA origami
Cancer immunotherapy has revolutionized the treatment of various malignancies. However, current therapeutic strategies often face limitations in precision, reproducibility, and control over immune cell activation. The LIPIRNADO project aims to address these challenges by developing DNA origami-based molecular platforms to precisely modulate T cell receptor (TCR) signaling. By mimicking natural spatial arrangements of immune ligands, this project explores how nanoscale geometry and molecular crowding affect T cell activation. The ultimate objective is to pave the way for programmable, synthetic immunotherapies with higher specificity and tunability. This fundamental research contributes to EU priorities in health, innovation, and responsible technology development.
Data: CORDIS, © European Union
Project objective
mRNA vaccines have gained considerable attentions in prevention and treatment of virus infections, cancer and genetic diseases, especially in the application to the COVID-19 pandemic in recent years. Due to the immunogenicity and instability of mRNA, the in vivo delivery of mRNA plays a crucial role in vaccine efficacy. Among various materials, lipid nanoparticles (LNPs) have shown to be one of the most potent carriers for mRNA delivery and successfully enter the clinic. To further improve vaccine efficacy, dendritic cells (DC) uptake can be optimized by decorating mannose ligands on LNP-mRNA formulations for targeted vaccine delivery. However, the effect of ligand density, spatial distance and organization pattern on cellular uptake remains poorly understood. Due to the addressability of DNA origami, nanoparticles and small molecules can be spatially organized into prescribed pattern with nanoscale precision. Therefore, we propose to develop designed ligand patten on LNP-mRNA formulations by transferring ligand pattern distributed on DNA origami to LNPs. With ligands modified on surface, LNP-mRNA formulations will be used to investigate the effect of ligand pattern on DC uptake, which is not accessible to other targeted delivery system. The results of the project will promote the development of vaccine delivery field. In addition, it will influence the health care and biomaterial industries, which are central to Europe’s ongoing and future economic success.
Original text from CORDIS.
Participants
- KAROLINSKA INSTITUTET · STOCKHOLMCoordinatorSweden
Links
- View on CORDIS
- DOI: 10.3030/101068419
- https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e507c4bda3&appId=PPGMS
- https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e520e33001&appId=PPGMS
Data: CORDIS, © European Union