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HEDoctoral network2022–2026

greenerRPP · Greener, more sustainable platforms for high-value recombinant protein production

Horizon Europe — Marie Skłodowska-Curie Actions

Duration
2022-09-01 → 2026-08-31
EU contribution
€2,473,985
Participants
16
Scheme
HORIZON-TMA-MSCA-DN

Lines connect the coordinator with its partners.

Results in brief

Greener, more sustainable platforms for high-value recombinant protein production

The overall objective of GreenerRPP research is to improve the sustainability and economic viability of industrial protein production in the bacteria Escherichia coli and Bacillus subtilis, and CHO cell lines, and to enhance both product quality and quantity with particular focus on the greener and more sustainable production of recombinant proteins. To realise the main objectives, four interlinked Work Packages (WPs) address the key problems in this field: WP1 aims at the development of new strategies to express target proteins in the three selected host chassis, using a series of innovations to: (i) produce such proteins in high amounts in the cytoplasm of E. coli and B. subtilis; (ii) export them to the periplasm or growth medium in E. coli and B. subtilis and (iii), secrete high amounts of biotherapeutics from CHO cell lines. WP2 serves as the ‘strain clinic’ to improve the host strains by counteracting the stress and adaptive responses encountered under WP1. WP3 is aimed as a “bridge” between WP1 and WP2, by integrating the acquired data with previously published data on stress responses and construct computational models that identify mitigation strategies. WP4 is aimed at benchmarking the new strains for industrial applications.

Data: CORDIS, © European Union

Project objective

The greenerRPP project aims to markedly enhance the EU’s capacity for the production of secreted high-quality recombinant proteins, especially industrial enzymes and therapeutic proteins (biotherapeutics). The combined markets are in excess of $330 billion p.a., and the products are of massive significance for healthcare and a sustainable bio-based economy. greenerRPP will address urgent problems in these industries, such as difficult-to-express proteins and the rapidly-emerging issue of 'sustainability' in the European biotechnology industry. It will deliver improvements in yield and process design that increase the industry's effectiveness while simultaneously reducing high current levels of waste. It will deliver industrial enzymes that reduce energy and raw material costs in countless industrial and domestic settings. To achieve this, we will develop powerful new microbial and mammalian cell platforms that produce recombinant proteins in unusually high quantities and in unparalleled quality, and bring to market a suite of super-producing platforms. A comprehensive training programme will equip greenerRPP ESRs with the interdisciplinary and intersectoral skills required to be future leaders in these industries, while emphasising sustainability and gender-diversity dimension principles at every level. Finally, the programme addresses the stark global inequality in this biotechnology sector: recombinant protein production is largely carried out in (or outsourced by) high-income countries, placing low/middle income countries at a massive disadvantage. greenerRPP includes a series of South East Asian Institutions as Associated Partners, to develop a global network with a knowledge exchange-secondment programme that boosts these countries' own manufacturing programmes. greenerRPP ESRs will thus enhance a European industrial sector that has never been more important to society, while making it 'greener' and reaching out to lower income countries.

Original text from CORDIS.

Participants

  • ACADEMISCH ZIEKENHUIS GRONINGEN · GroningenCoordinatorNetherlands
  • AB ENZYMES GMBH · DARMSTADTGermany
  • BOEHRINGER INGELHEIM RCV GMBH & CO KG · WienAustria
  • DSM FOOD SPECIALTIES BV · DelftNetherlands
  • EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH · ZuerichSwitzerland
  • EV BIOTECH BV · GroningenNetherlands
  • FGEN AG · BaselSwitzerland
  • KING MONGKUT'S UNIVERSITY OF TECHNOLOGY THONBURI · BangkokThailand
  • MAHIDOL UNIVERSITY · NAKHON PATHOMThailand
  • NATIONAL INSTITUTE FOR BIOPROCESSING RESEARCH AND TRAINING LIMITED · BLACKROCK DUBLINIreland
  • NATIONAL INSTITUTE OF VETERINARY RESEARCH · HANOIVietnam
  • OULUN YLIOPISTO · OuluFinland
  • UNIVERSITAET GREIFSWALD · GreifswaldGermany
  • UNIVERSITATSMEDIZIN GREIFSWALD KORPERSCHAFT DES OFFENTLICHEN RECHTS · GreifswaldGermany
  • UNIVERSITI MALAYSIA SABAH · Kota KinabaluMalaysia
  • UNIVERSITY OF KENT · Canterbury, KentUnited Kingdom

Links

Data: CORDIS, © European Union