DUNE · Deep eutectic solvents for membrane transport of nucleic acids
Horizon Europe — Marie Skłodowska-Curie Actions
- Duration
- 2023-09-01 → 2025-08-31
- EU contribution
- €165,313
- Participants
- 1
- Scheme
- HORIZON-TMA-MSCA-PF-EF
Lines connect the coordinator with its partners.
Results in brief
Deep eutectic solvents for membrane transport of nucleic acids
The Action DUNE – Deep Eutectic Solvents for Membrane Transport of Nucleic Acids looks at how solvent engineering can be exploited to stabilise nucleic acids and transport those across biological membranes. The delivery of genetic material as therapeutics constitutes one of the most promising approaches to treat emergent pathogens and important diseases. However, the current technologies are threatened by the low stability of the nucleic acids, the limited efficacy of transport across cell membranes, and the relatively high toxicity of the formulations. DUNE aimed to create a groundbreaking approach for the development of deep eutectic solvents (DESs) as a formulation platform for the preservation and cytosolic delivery of nucleic acids. DESs are non-aqueous solvents formed through mixing simple organic molecules with the capacity to solubilise and stabilise biomolecules. The large number of possible combinations of precursors for the preparation of DESs yields a tailorable platform, in which the properties of the solvent can be optimised for the functional stabilisation of nucleic acids. Potentially, this tailorable character can be exploited to develop suitable environments for nucleic acids with the capacity to transport them across biological membranes.
Data: CORDIS, © European Union
Project objective
The delivery of nucleic acids as therapeutic methods holds great potential to provide new treatments against emergent pathogens and important diseases. However, the development of functional therapies is often hindered by the poor efficiency and stability of the current transport technologies. Deep eutectic solvents (DES) are non-aqueous sustainable liquids obtained through the combination of simple organic molecules. The physicochemical properties of the DES can be tailored through the selection of their constituents, to optimally perform in specific applications. DES have been recently shown to increase the stability and activity of nucleic acids (NA), as compared to those in aqueous systems. Despite the excellent and encouraging physicochemical properties of DES, these solvents have not been investigated, to date, for the transport of NA across cell membranes. This project will finally fill the knowledge gap that exists between the promising properties of DES and their potential ability to selectively deliver NA into the cell cytosol. Here, a combinatorial approach will be used to synthesise a library of DES with different properties relevant for transport (e.g., amphiphilicity and charge density). The ability of these to enable membrane transport of NA will be investigated as a function of the system properties. The exclusive properties of the DES solvent will go beyond the state-of-the-art, as they will allow: i) fine-tuning of DES/cargo interactions and binding strength by DES counterion adjustment; ii) precise hydrophobic DES control for membrane partition regulation; iii) enhanced physicochemical stability of the resulting particles and simpler synthetic and scaling up methodology, as well as reduced production costs. Overall, this project is aimed to discover a completely new range of potential applications of DES in membrane transport and biomolecular cargo preservation, which will open a new research field of potential applications in NA delivery.
Original text from CORDIS.
Participants
- UNIVERSIDAD DE SANTIAGO DE COMPOSTELA · Santiago De CompostelaCoordinatorSpain
Links
- View on CORDIS
- DOI: 10.3030/101063372
- https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e50d300994&appId=PPGMS
Data: CORDIS, © European Union