ImageTumor · X-Ray Fluorescence Imaging of Metal Complexes in Hypoxic Tumor Spheroids
Horizon Europe — Marie Skłodowska-Curie Actions
- Duration
- 2023-06-01 → 2025-05-31
- EU contribution
- €189,687
- Participants
- 2
- Scheme
- HORIZON-TMA-MSCA-PF-EF
Lines connect the coordinator with its partners.
Results in brief
X-Ray Fluorescence Imaging of Metal Complexes in Hypoxic Tumor Spheroids
Context According to estimates from the World Health Organization (WHO) in 2020, cancer is the second leading cause of death with approximately 10 million deaths. The field of metal-based anticancer drugs has been on the rise ever since the discovery of the anticancer properties of cisplatin in 1965, which is in daily clinical use against various types of cancer. Many compounds failed to enter clinical studies since their anti-cancer activity was mainly studied in two-dimensional (2D) monolayer cells which does not mimic the complex tumor microenvironment in humans. In the human body the cancer tumours grow in 3-dimensional structure, hence it is vital to study the anticancer activity of a drug molecules in the 3D tumours rather in 2D mono layer cells. We have investigated the structure activity relationship of Rhenium-metal complexes to realise the effect of the cytotoxicity in 3D tumor spheroids. Key objectives of the project 1. Synthesis and characterization of Re-metal complexes. 2. Study the cytotoxicity in 2D and 3D tumor spheroids and compare and investigate the cell death mechanism. 3. Investigate the cellular uptake and localization in cancer cells with confocal fluorescence microscopy where applicable.
Data: CORDIS, © European Union
Project objective
According to estimates from the World Health Organization (WHO) in 2019, cancer is the second leading cause of death after heart failure before the age of 70. The field of metal-based anticancer drugs has been on the rise ever since the discovery of the anticancer properties of cisplatin in 1965, which is in daily clinical use against various types of cancer. Many compounds failed to enter clinical studies since their anti cancer activity is mainly studied in two dimensional (2D) monolayer cells which does not mimic the complex tumor micro environment in humans. In the human body the cancer tumors grow in 3-dimensional structure, hence it is vital to study the anticancer activity of a drug molecules in the 3D tumors rather in 2D mono layer cells. Next, tumor hypoxia (1% Oxygen), a situation where tumor cells are deprived of oxygen, is a major challenge for oncology. In this project we propose the synthesis of N-Heterocyclic Carbene/1,8-naphthalicanhydride conjugated series of metal complexes (Gold, Rhodium, Iridium) to study the anticancer activity and penetration depths in 3D tumor spheroids under hypoxia. We also investigate the activation by reduction mechanism in presence of cellular thiols and cytotoxicity in vitro (3D cell spheroids) and compare the results among the three metal centers. The metal protein interactions play key role in many biological processes; hence we also investigate the metal-protein interaction studies using capillary electrophoresis inductively-coupled mass spectrometry (at secondment). We will investigate the structure activity relationship between metals and of related coordinating ligands in 3D spheroid under hypoxic conditions. We mainly utilize X-Ray Fluorescence (XRF) imaging and synchrotron radiation to test the cellular localizations and penetration depth for the proposed metal complexes. This project is expected to yield unique insights towards optimization of next-generation anti-tumor drugs.
Original text from CORDIS.
Participants
- RUHR-UNIVERSITAET BOCHUM · BochumCoordinatorGermany
- THE UNIVERSITY OF AUCKLAND · AucklandNew Zealand
Links
- View on CORDIS
- DOI: 10.3030/101066639
- https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e50a8dfc36&appId=PPGMS
- https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e51bfbd779&appId=PPGMS
Data: CORDIS, © European Union