BIO DEGRADE · Bacteria Intrinsically Orchestrated with Designed Enzymes for sinerGistic Rate-Accelerated plastic Degradation Efficiency
Horizon Europe — Marie Skłodowska-Curie Actions
- Duration
- 2023-04-01 → 2025-09-30
- EU contribution
- €226,441
- Participants
- 3
- Scheme
- HORIZON-TMA-MSCA-PF-EF
Lines connect the coordinator with its partners.
Results in brief
Bacteria Intrinsically Orchestrated with Designed Enzymes for sinerGistic Rate-Accelerated plastic Degradation Efficiency
Plastic pollution is a major global environmental threat. Annually, over 400 million tonnes are produced, with less than 10% recycled effectively. The breakdown of plastics creates microplastics, found everywhere and harmful to life. Current recycling methods struggle with the sheer volume and complexity of plastic waste. Biotechnology offers a promising, sustainable alternative through enzymes and microbes. The BIODEGRADE project aimed to create a microbial system to break down and upcycle polyethylene terephthalate (PET), a common plastic. Our main goal was to genetically modify microorganisms to express their own proteins engineered to be capable of degrading PET. By combining computer-based protein design and synthetic biology, we sought to develop efficient biological catalysts that work together to degrade plastic without harming the host bacteria, ultimately creating new bacterial strains for plastic bioconversion. This addresses the urgent need for sustainable waste solutions and a circular bioeconomy, aligning with the European Green Deal and the Circular Economy Action Plan. BIODEGRADE results are expected to influence industrial practices and environmental policy by enabling scalable biological recycling. This research also promises socioeconomic benefits by supporting green jobs and sustainable manufacturing.
Data: CORDIS, © European Union
Project objective
Plastics are one of the most widely used materials, with a wide variety of applications across all sectors of the economy. Its production is estimated to be around 360 million tonnes, and thus synthetic polymer is poorly biodegradable. Plastic life cycle is responsible for greenhouse gas emissions, contributing to climate change, plastic waste has been found in every ecosystem, are broken down into small pieces, microplastics, which can have a negative impact on organisms, and altogether causing an unprecedented ecological crisis. We aim to tackle this problem through Bacteria Intrinsically Orchestrated with Designed Enzymes for sinerGistic Rate-Accelerated plastic Degradation Efficiency (BIO DEGRADE) project, using a novel application of biotechnological-based approaches to synergistically increase the efficiency limits of multi-enzymatic solutions. The project will perform genomic modification of microorganisms with their own evolved collection of computationally-engineered proteins for (micro)plastic degradation. We are not engineering single enzymes to enhance their catalytic activities, as it will computationally design multitude (hundreds) of proteins from the microorganism, and boost them with extra catalytic sites making them into plurizymes. The additional active sites able to degrade plastics will designed into the proteins employing advanced molecular simulation techniques such Protein Energy Landscape Exploration (PELE) together with machine learning methods. In this way, the proteins will not be from other microbial origin (heterologous), but their homologous proteins, to which an extra active-site capable of hydrolysing microplastics will be designed and genetically added, not altering the gene expression pattern of the microorganisms. These organisms will outperform the use of single enzymes because of the synergistic action of multiple evolved enzymes with one common goal: (micro)plastic degradation.
Original text from CORDIS.
Participants
Links
- View on CORDIS
- DOI: 10.3030/101104264
- https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e501fcc336&appId=PPGMS
- https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e5217fc934&appId=PPGMS
Data: CORDIS, © European Union