FLUO.OPC · Fluorescence-based optical phase conjugation through scattering media

Fluorescence-based optical phase conjugation through scattering media

The ability to generate and control light through complex media such as thick biological tissue is essential for optical microscopy and many biomedical applications. However, strong scattering within such media severely limits our capacity to manipulate light. Recent advances in wavefront shaping have made progress in addressing this challenge, but these methods rely on coherent laser light and thus cannot be applied to fluorescence, which underpins modern microscopy techniques. Expanding the ability to focus or deliver light to multiple or extended fluorescent targets would greatly broaden the range of applications, particularly in deep-tissue optogenetics, where fluorescent probes enable precise and targeted labeling. This project aims to achieve active control of light propagation through scattering media by exploiting fluorescence signals from fluorescent objects. By combining advanced holographic techniques to extract information from fluorescence with optical phase conjugation, the project seeks to demonstrate that light can be accurately focused through thick, highly scattering media guided by fluorescence.

Data: CORDIS, © European Union