Monitoring calcium concentration in the endoplasmic reticulum using recombinant aequorin specifically targeted
FP4 — Training and Mobility of Researchers
- Duration
- 1996-03-01 → 1997-02-28
- EU contribution
- —
- Participants
- 2
- Scheme
- RGI
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Project objective
The project proposed constitutes an extension of that carried out during my stay in the University of Padova. I will first construct a set up to measure aequorin luminescence, similar to those actually running in Padova. Then, I will use the HeLa clones I have obtained in Padova, which stably express the DNA construct designed to target aequorin (both native and mutated with low Ca2+ affinity) to the endoplasmic reticulum (ER). These cells will be used to continue the study of ER-Ca2+-heterogeneity currently being carried out in Padova. At the same time, I plan to produce a series of point mutations in the Ca2+ -binding sites of aequorin in order to reduce its Ca2+-affinity up to near the mM range. In the mean time, Sr2+ will be used as a low-affinity Ca2+-surrogate. Sr2+ has been shown to behave similarly to Ca2+ in terms of uptake, accumulation and release from the ER.
Original text from CORDIS.
Participants
Links
Data: CORDIS, © European Union