Protein protein and dna protein transactions during gene activation activator sigma n contacts and the dna melting activity of the sigma n activator complex

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Project objective

Determination of the critical protein-protein interactions in transcription systems and in particular between sigma-N and its activator, and the contribution of the sigma-N holoenzyme-activator complex to the DNA strand separation (melting) process are essential for the understanding of the mechanism of transcriptional activation of the RNA polymerase holoenzyme. The sigma-N protein is well suited to analysis as biochemically active domains have been isolated. The overall aims of the proposed work are to: (a) Locate the putative protein-protein interactions between activator and the sigma-N protein, that appear to be critical for activation of RNA polymerase holoenzyme. (b) Identify promoter DNA-contact regions of the sigma-N holoenzyme-activator complex during catalysis of open complex formation. (c) Mutate candidate DNA and activator contact regions of sigma-N identified as described above. Screen mutants defective in either a DNA contact during DNA melting and/or activation interaction. (d) Analyse the DNA-melting process, dependent of the formation of the sigma-N holoenzyme-activator complex.

Original text from CORDIS.

Participants

• IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE · LONDONCoordinatorUnited Kingdom
• Not availableCity levelSpain

Links

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