Studies on the phloem specificity of the coconut foliar decay virus promoter
FP4 — Training and Mobility of Researchers
- Duration
- 1996-10-01 → 1997-09-30
- EU contribution
- —
- Participants
- 2
- Scheme
- RGI
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Project objective
Coconut foliar decay virus (CFDV) is a small single-stranded DNA virus which is limited to phloem tissues of coconut palm. A viral promoter has been identified which, if stably transformed into tobacco in fusion with GUS, shows GUS activity confined to the primary and secondary phloem tissue. I propose to map the phloem-specific regulatory elements in the promoter region by site- directed mutagenesis followed by in situ localization of GUS activity in transformed tobacco. This work will be completed by foot-printing and mobility-shift experiments with tobacco nuclear protein extracts to identify protein-binding regions within the promoter. These regions will be used as probes to screen a tobacco expression library for the corresponding proteins.
Original text from CORDIS.
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Data: CORDIS, © European Union