A gene trap screen for the identification of genes involved in motoneuron development
FP4 — Training and Mobility of Researchers
- Duration
- 1996-05-01 → 1998-04-30
- EU contribution
- —
- Participants
- 2
- Scheme
- RGI
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Project objective
My aim is to dissect the molecular events leading to the differentiation of a specific sub-class of neurons i.e. motoneurons, by using the recently-developed gene trapping technology to identify genes which are specifically transcribed in cells which are differentiating toward a motoneuron phenotype. 1. A replicate bank of embryonic stem cell clones will be generated containing random integrations of the B- galactosidase reporter gene into the mouse genome. 2. Individual ES cell clones will be induced to differentiate in vitro towards a motoneuron phenotype using conditions recently identified in the host laboratory. Clones of interest will be identified by a B-gal assay and further characterised using molecular genetic techniques. 3. These clones will be used to generate transgenic mice in which the in vivo expression pattern of the B-gal (and thus of the trapped gene) as well as the effects on motoneuron development of null mutations in the trapped genes can be analysed.
Original text from CORDIS.
Participants
Links
Data: CORDIS, © European Union