A new model for rna polymerase/promoter interaction in escherichia coli

Lines connect the coordinator with its partners. CORDIS does not always give exact coordinates for projects before 2014. These points are placed at city or country level.

Project objective

In E. coli, most transcription activators work by making contacts with either the a subunit of RNA polymerase (Class-l transcription activators) or with the cs factor (Class-IU). The Ada protein, a transcriptional activator for a set of genes involved in repair of DNA alkylation damage, has been identified as a Class-l activator, since deletions in the C-terminal domain of the a subunit severely impair Ada activation at the ada promoter. We have shown that the C-terminal domain of a is in fact responsible for binding of RNA polymerase to the promoter region of the ada and the aidB genes. This observation suggests that the role played by the a subunit of RNA polymerase at these promoters is to interact with the promoter DNA rather than with the transcriptional activator Ada. This possibility would suggest a novel mechanism for RNA polymerase interaction with positively controlled promoters. In this proposal, we outline a series of experiments to determine if a-mediated binding of RNA polymerase to the ada and aidB promoters is necessary for Ada-activated transcription.

Original text from CORDIS.

Participants

• UNIVERSITY OF BIRMINGHAM · BIRMINGHAMCoordinatorUnited Kingdom
• Not availableCity levelItaly

Links

• View on CORDIS