The characterisation of a putative human cell division cycle gene and study of its possible role in intestinal differentiation

Lines connect the coordinator with its partners. CORDIS does not always give exact coordinates for projects before 2014. These points are placed at city or country level.

Project objective

During my present research on the intestinal enzyme lactase I have found a putative human cell division cycle gene. I wish to follow up this initial finding, and isolate and characterise a complete cDNA corresponding to this gene. This will enable the analysis of the relationship of this transcript to putative homologues identified in yeast and other organisms. I propose to investigate the pattern of expression using RT PCR, Northern blotting and in situ hybridisation techniques. I also aim to make specific antibodies for analysis of the resulting polypeptide. I will study cell lines which are being used as models for intestinal differentiation in addition to cell lines in which the cell cycle call be regulated by drug treatments. I also aim to clone and characterise the promoter region of this gene to enable future studies on the regulation of expression.

Original text from CORDIS.

Participants

• Ayuntamiento de Barcelona · BarcelonaCoordinatorSpain
• Not availableCity levelUnited Kingdom

Links

• View on CORDIS