Role of the cytoplasmic tail of hiv and siv in envelope expression and virus release

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Project objective

Research objectives and content The envelope glycoproteins of the human and simian immunodeficiency viruses (HIV and SIV) are responsible for cell infection by mediating virus binding and entry. It is known that they are also important for late events in the replicative cycle in particular the assembly, budding and release of newly synthesised virions. It has been suggested that the cytoplasmic tail of the transmembrane component of the envelope glycoprotein can modulate the cellular location, cell surface expression and incorporation of envelope into viral particles. I propose to characterise the features of the protein that mediate these effects and investigate the interactions between the envelope glycoprotein cytoplasmic domain and other viral and cellular proteins. This will be done by analysing the intracellular transport of newly synthesised envelope glycoproteins and locating the sites of viral budding. I will perform morphological and biochemical analyses on the envelope glycoproteins (and mutants of these proteins) expressed in T cells and macrophages alone, or together with other viral structural proteins. For these studies, I will benefit from the expertise of the host institution in the cell biological analysis of virus-cell interactions, the use of novel expression techniques (e.g. Semliki Forest virus) and the use of green fluorescent protein as a means of protein tagging. Training content (objective, benefit and expected impact) I anticipate this project will lead to a clearer understanding of the assembly of HIV and SIV, and a better understanding membrane protein sorting mechanisms in general. Links with industry / industrial relevance (22)

Original text from CORDIS.

Participants

• UNIVERSITY COLLEGE LONDON · LONDONCoordinatorUnited Kingdom
• Not availableCity levelFrance

Links

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