Characterization of viral and host proteins involved in actin tails formation during vaccinia virus infection
FP4 — Training and Mobility of Researchers
- Duration
- 1997-02-01 → 1999-01-31
- EU contribution
- —
- Participants
- 2
- Scheme
- RGI
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Project objective
Research objectives and content Recently, vaccinia virus has been shown to move within the host cell and from cell to cell using actin polymerization as a driving force (Cudmore et al., 1995, Nature 378, 636-638; Cudmore et al., 1996, J. Cell Sci., In press). This project aims to identify the viral protein that initiates the cascade of events that lead to the polymerization of the actin tail that propels the virus particle as well as the host factors involved in this process. Actin tails are only induced by the IEV (intracellular enveloped virus) form of vaccinia, whose outer membrane surface is derived from the trans Golgi network (TGN). Through a combined screen and biochemical approach, we will identify the viral protein present at the IEV surface that is responsible for initiating the cascade of events that lead to actin tail formation and ultimately movement. In addition, we will develop in vitro assays using cell extracts from a variety of sources to facilitate identification and study of both viral and host proteins involved in the actin based motility of vaccinia virus. training content (objective, benefit and expected impact) The main objective is the understanding the spread of vaccinia as a model to understand the spread of viruses. Taking advantage of my yeast actin cytoskeleton background, the post-doctoral project described here will permit my entrance into the domain of mammalian cytoskeleton and viral pathology. I will benefit from training in the handling of viruses and tissue culture as well as in in vitro assays to study motile processes. EMBL will also be the perfect place to learn new techniques such as electronmicroscopy. Identification of viral and host factors involved in the motile process will be an important first step in the molecular characterization of the role of the cytoskeleton in viral infection. Links with industry / industrial relevance (22)
Original text from CORDIS.
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