Identification of fgf-stimulated signalling pathways critical for angiogenesis

Lines connect the coordinator with its partners. CORDIS does not always give exact coordinates for projects before 2014. These points are placed at city or country level.

Project objective

Research objectives and content The aim is to identify the signal transduction pathways critical for FGF-mediated angiogenesis. The novel immortomouse brain endothelial (IBE) cell line, offers the to potential to disect the pathway critical for FGFmediated tube-formation. Using the innovative approach of transfecting these cells with PDGFR-alpha/FGFR-1 chimeric receptor mutations via retrovirus-mediated gene transfer, I will study the ability of specific mutations to inhibit tube-formation. I will then determine which signal transduction cascades have been perturbed. Furthermore, I will also utilise a yeast two-hybrid system to identify novel proteins which associate with the intracellular domain of the FGFR-1. The biological role of such proteins will then be identified. Training content (objective, benefit and expected impact) I will be trained in a wide range of current molecular biology techniques. Also, I will learn a number of biological assays, critical for studying angiogenesis in a cell model. The benefit of such training is that I will be learning a wide range of skills critical to my scientific development. Since the host lab has renown record in this field I expect to generate novel data. Links with industry / industrial relevance (22) Identification of pathways critical for angiogenesis will facilitate the development of agents which can regulate such pathways. A pharmaceutical involvement is highly anticipated.

Original text from CORDIS.

Participants

• UPPSALA UNIVERSITY · UPPSALACoordinatorSweden
• Not availableCity levelUnited Kingdom

Links

• View on CORDIS