Comparison of regulatory sequences of polyhomeotic and its human homologs inchromatin activity in drosophila

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Project objective

Research objectives and content Objective A: To develop improved site specific recombinase based strategies for targetted chromosomal integration of DNA in Drosophila. This will enable the problem of genomic position effects in the analysis of chromatin mediated regulation to be overcome. Objective B: To use the above targetting strategy for the comparison, at the same integration locus,of regulatory regions of the Drosophila gene polyhomeotic and its human homologs.The isolation and characterisation of higher eukaryotic homologs of Drosophila Polycomb group (PcG) and trithorax group (trxG) genes has shown that chromatin mediated mechanisms of silencing and activation appear to be broadly conserved throughout evolution. In Drosophila, the polyhomeotic gene, itself a member of the PcG, is regulated by PcG and trxG products. I propose an analysis of the regulatory regions of human homologs of po-eotic (HPHI and HPH2), and comparison with those of the Drosophila gene, based on assays in Drosophila for chromatin silencing and activation. Training content (objective, benefit and expected impact) I will learn techniques in Drosophila genetics and the analysis of chromatin activity in Drosophila. Benefit / impact This new training in combination with my experience in biochemistry and molecular biology, and my familiarity with site specific recombinase technology, will give me a powerful range of skills with which to study mechanisms of chromatin regulation. Links with industry ! industrial relevance (22)

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Participants

• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · PARISCoordinatorFrance

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