Mechanisms of cell cycle exit regulation by the retinoblastoma-related protein p130

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Project objective

The aim of this proposal is to study protein interactions specific of p130 phosphorylated forms occuring upon cell cycle exit, in order to ascertain novel mechanisms that integrate growth inhibiting and/or differentiating signals and that may be involved in cell transformation when altered. The research strategy will consist in the use of different p130 mutants, involving specific protein domains and phosphorylation sites, as baits in yeast two-hybrid screening assays. The cDNA libraries to be screened will be obtained from particular conditions of cell cycle exit where preliminary work indicates a primary function of p130. The characterise of p130 mutants, of the cDNA clones obtained, and the study of cellular interaction of individual p130 phosphorylated forms will provide a mean of ascertaining the precise role of p130 during cell cycle exit. The training objective of this proposal is to complement my previous research formation with the leading experience of the host laboratory in some aspects of the cell cycle field, in order to conduct independent research in the future. The expected results will be instrumental in understanding processes of cell cycle exit, and they may have relevant implications defining novel mechanisms of cell transformation. The host laboratory is linked to the european biotechnology company Prolifix Results arising from this proposal will be used for application in drug design a production if suitable (see Description of the Host Institute).

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Participants

• Aristotle University of Thessaloniki · ThessalonikiCoordinatorGreece

Links

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