Search for genes interacting with irrec-rst in vivo

Lines connect the coordinator with its partners.

Project objective

Research objectives and content The Drosophila irregular chiasm C - roughest (irreC-rst) gene encodes a member of the DM-GRASP subgroup of the neural cell adhesion molecules of the immunoglobulin superfamily. The main aim of this project is to gain a better understanding of the biological function of IrreC-rst protein through identification of interacting partners. We plan to realise genetic screens in order to search for mutations second-site that enhance or suppresse mutant phenotypes using sensitized genotypes of irreC-rst gene. We expect from such screens to be able to isolate functionally interacting genes and to elucidate their hierarchy in regulatory pathways. Analysis of mosaic animals after mitotic recombination could permit to investigate the phenotype of the mutant clones in wild-type or otherwise mutant imaginal discs. Study of IrreC-rst expression in different mutant backgrounds could unravel interactions at the level of transcription. Another part of the proposal project is a genetic analysis of several truncated IrreC-rst proteins in vivo. Training content (objective, benefit and expected impact) Members of the DM-GRASP subfamily of neural cell adhesion molecules are temporally and spatially highly regulated and assumed to function as neuronal identifiers during brain development and l neuronal regeneration. So far knock-out and gain of function mutant phenotypes are only known for the IrreC-rst protein in Drosophila. They support the assumed significance of this class of proteins. The identification of the underlying molecular interactions will be helpful to understand regulatory pathways in which a member of the DM-GRASP subfamily of neural cell adhesion molecules is involved. Links with industry / industrial relevance (22)

Original text from CORDIS.

Participants

• Aristotle University of Thessaloniki · ThessalonikiCoordinatorGreece

Links

• View on CORDIS