Carbon nutrition and metabolism in arbusacular mycorrhizas as affected by increasing temperature and atmospheric co2

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Project objective

The main objectives are:1) To study the effects of temperature (T) on carbon (C) metabolism and translocation in arbuscularmycorrhizas (AM), to determine if low temperatures limit the development of the extrardical mycelium to a larger extent than the intraradical mycelium due to a reduced mobility of lipid bodies, a decrease in C transfer from the plant, in the synthesis of triacylglycerides, or in the formation of lipid bodies; and2) To explore C limitation in the nutrition of AM fungi to determine ifi) plant C supply precludes at any time AM fungi development or function andii) AM fungi's response depends on their C and N status and/or developmental stage.The effects of T and CO_2 on C nutrition and metabolism in different isolates of AM fungi will be studied in 3 experimental systems simultaneously scaling up in complexity and reality: monoxenic root organ cultures, monoxenic whole plant cultures and plant-soil microcosms, where C will be followed by means of microscopy and microscopy data analysis, stable isotopic C labelling followed by nuclear magnetic resonance (NMR) and biochemical analysis of lipids, and radioactive C labelling and scintillation counting. This information is crucial for accurate prediction of C movement into the soil and realistic C budgets.

Original text from CORDIS.

Participants

• CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS · MADRIDCoordinatorSpain

Links

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