Comparative genome organisation in leptosphaeria maculans and related filamentous fungi

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Project objective

Position available for doctoral fellows in the fields of genomics and marker development in filamentous fungi. The following topics can be addressed: (i) Contribution to the analysis of genome organization of L. maculans. Following characterization of L. maculans or heterologous EST s (including candidate genes from other fungal species) , search for functional clustering by hybridization on the SAC library. Genome location of these clusters in the physical map and chromosomes separated by PFGE. Relationship with the location of known genes of L. maculans, including A VR genes.(ii) Comparison with the genome organization of related pathogenic fungi, by analyzing the occurrence, or not, of similar stretches of OAFs upon the availability of similar material and tools in the fellow host lab.(iii) Use of mini satellites as tools for population genetics or epidemiology in L. maculans or related fungal species. The availability of numerous sequence data in L. maculans, or cloning strategies targeted at markers linked in repulsion enabled us to design strategies to generate minisatellites. These single-locus sequences are common in fungal genomes and have the main advantage over microsatellites that they may be easily separated and analyzed using regular agarose electrophoresis due to the differences in size from one repeat to the other. New minisatellites will be characterized and their usefulness as markers assessed on natural L. maculans populations. Similar strategies can be developed on related fungal species as in (ii).

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Participants

• INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE · PARISCoordinatorFrance

Links

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