GLOW-FISH · Visualisation and analysis of exo- and endocytosis events at neuronal synapses of the zebrafish

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Project objective

The basic aim of this proposal is to investigate the molecular mechanisms of fast and slow endocytosis at the synapse. We will generate stable lines of zebrafish expressing fluorescent proteins thought to be involved in endocytosis under control of neuron al promoters; then use TIRFM to monitor recruitment of these proteins to the presynaptic membrane in the giant synaptic terminal of retinal bipolar cells. In particular, we will produce zebrafish lines expressing the fluorescent fusion proteins clathrin-l ight-chain-a-monomeric red fluorescent protein (clathrin-mRFP), endophilin-mRFP, amphiphysin-mRFP and synaptopHluorin a pH sensitive GFP related reporter of exocytosis and endocytosis. We will test the hypothesis that endophilin is involved in fast endocyt osis at the synapse, while clathrin and amphiphysin mediate slow but not fast endocytosis. These transgenic zebrafish lines may also be used to monitor exocytosis and endocytosis in intact neural circuits, such as the retina, using multi-photon confocal mi croscopy.

Original text from CORDIS.

Participants

• MEDICAL RESEARCH COUNCIL · LONDONCoordinatorUnited Kingdom

Links

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