STARTSITE · Structural studies of the RNA polymerase II transcription machinery: mechanism of start site selection
FP6 — Marie Curie Actions (Human Resources and Mobility)
- Duration
- 2005-04-01 → 2008-03-31
- EU contribution
- €253,429
- Participants
- 2
- Scheme
- OIF
Lines connect the coordinator with its partners. CORDIS does not always give exact coordinates for projects before 2014. These points are placed at city or country level.
Project objective
The current application concerns the mechanisms of transcription initiation in eukaryotic cells. The postdoc-project will be carried out in Prof. Roger D. Kornberg's laboratory at Stanford University, California.THE SPECIFIC AIM of the project is a molecular understanding of how RNA polymerase II (RNAP II) together with the general transcription factors (GTFs) can recognize promoter DNA and initiate RNA synthesis at a specific genomic location.Reports have shown that RNAP II and TFIIB are solely responsible for this process, but the underlying mechanism is still not known. Recently the structure of a RNAP II/TFIIB cocrystal has been solved in budding yeast. The transcription start site differs however in this yeast compared to fission yeast and most other eukaryotes including human so a general model for the selection process could not be elucidated from this structure.OUR MAIN OBJECTIVES are to do structural analysis of a RNAP II/TFIIB complex in fission yeast with X-ray crystallography and compare it to t he budding yeast structure. We will complement the structural information with mutational analysis of TFIIB in a pure in vitro transcription system derived from fission yeast developed by me as a Ph.D. student.THE OVERALL GOAL with the project is to generate a general molecular model for the transcription start site selection process.My stay will lead to the acquirement of new technological knowledge such as structural analysis of multi-protein complexes with X-ray crystallography that I believe will be a vibrant scientific field in the future.I will also be able to develop a scientific network that will be useful for my future career as a scientist, as well as the European community, after my return to Sweden.
Original text from CORDIS.
Participants
- KAROLINSKA INSTITUTET · STOCKHOLMCoordinatorSweden
- STANFORD UNIVERSITY SCHOOL OF MEDICINE · STANFORDCity levelUnited States
Links
Data: CORDIS, © European Union