LOWCHEMOSMAT · Rational design of long-wavelength chromo- and fluorogenic chemosensors and composite materials for ionic analytes
FP6 — Marie Curie Actions (Human Resources and Mobility)
- Duration
- 2006-09-01 → 2008-08-31
- EU contribution
- €149,722
- Participants
- 1
- Scheme
- EIF
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Results in brief
Final Activity Report Summary - LOWCHEMOSMAT (Rational design of long-wavelength chromo- and fluorogenic chemosensors and composite materials for ionic analytes)
In optical sensing of chemical species, the performance of a sensor strongly depends on selectivity, sensitivity and the suitability of the wavelength range of communication. Fluorescence methods are very sensitive. However, in many environmental and biochemical applications the matrix can auto-fluoresce or absorb the light used for probing the sensor's response. Working in the red / near-infrared (NIR) region of the spectrum for optical sensing can improve the sensitivity of assays, since biological matrices are optically silent above 650 nm. These facts, together with the current availability of cheap excitation sources for the red region of the spectrum, have recently fuelled the development of red / NIR probes for different analytical applications. In this project, a remarkable contribution to chemical, photophysical and mechanistical knowledge on new red/NIR fluorescent probes has been made. Several families of dyes have been investigated and characterized, and important and novel results have been achieved. For example, a series of highly emissive boron-dipyrromethene (BODIPY) dyes have been studied and successfully included in polymer nanoparticles, yielding highly emissive and photo / chemically stable beads for applications in fluorescence confocal microscopy or multiplexing. The new BODIPY dyes investigated include styryl-substituted and aromatic ring-fused BODIPYs (with phenantherene or dihydronaphthalene ring fusion) with quantum yields of fluorescence approaching 100 %. Other remarkable results include the development of a rubyrin with ever-red shifted spectra, that has been applied as optode for Hg(II) detection in water when included in a polyurethane film, or expanded porphyrins core-modified with different heteroatoms. On the other hand, the synthesis of (hemi) cyanine dyes has also been explored and a meso-cyclam-substituted cyanine chromophore has been employed as NIR probe for the detection of the citrate anion following a displacement assay. Additionally, and in order to get some insight into the response mechanism of functional meso-substituted heptamethine cyanines, quantum mechanical calculations and photophysical studies with several model compounds were carried out.
Data: CORDIS, © European Union
Project objective
In optical sensing of chemical species, the performance of a sensor strongly depends on selectivity, sensitivity and the suitability of the wavelength range of communication. In many environmental and biochemical applications however the matrix can autofluoresce or absorb the light used for probing the sensor and response.A suitable way to circumvent such interferences and to improve the sensor and specificity is to operate in the red-visible or near infrared (NIR) spectral region, preferably with a sensitive and versatile method such as fluorometry. Biological matrices are optically silent above 650 nm. However, despite the rapid growth of the field of chemosensor research lately, examples of molecular NIR probes and actual sensor materials a re still very limited.The proposal aims to develop, characterize and test new functional molecules and composite sensor materials for the optical detection of a charged inorganic and a group of organic analytes of biological importance in these long-wave spectral ranges. To achieve these objectives, first, rational molecular design employing theoretical and empirical methods is used to generate suitable dye platforms with integrated receptor units that respond to a guest by specific changes in their spectroscopic signal.After synthesis and testing, the molecular probes are then incorporated into nanoscopic materials. The latter step allows tuning the selectivity and promises to obtain composite, organic-inorganic, sensor materials with tailor-made performance in an analytically advantageous spectral range.The proposal addresses many issues of FP6 and NMP Priority Areas 3.4.2.1-2 and 3.4.2.3-1 and thus lies in a highly topical research field. Its general scientific and technological work is assumed to generate new ideas on advanced chemosensor design and promises to achieve the development of innovative optical materials in nanotechnology.
Original text from CORDIS.
Participants
- BUNDESANSTALT FUER MATERIALFORSCHUNG UND -PRUEFUNG · BERLINCoordinatorGermany
Links
Data: CORDIS, © European Union