NANOTOX · Nano-particle characterization and toxicity
FP6 — Marie Curie Actions (Human Resources and Mobility)
- Duration
- 2006-12-01 → 2008-11-30
- EU contribution
- €180,134
- Participants
- 1
- Scheme
- IIF
Lines connect the coordinator with its partners. CORDIS does not always give exact coordinates for projects before 2014. These points are placed at city or country level.
Results in brief
Final Activity Report Summary - NANOTOX (Nano-Particle Characterization and Toxicity)
During the fellowship period, the Fellow worked with several researchers on projects aimed to determine the toxicity of nano-particles and to what extent the observed responses may be related to particle-induced reactive oxygen species (ROS). Particle-generated ROS may be one of the major mechanisms controlling the development of diseases' caused by inhalation of low-solubility particles. The Fellow worked on: 1) the development of ROS detection methods and their application in systems with and without cells; 2) studies to determine whether sample preparation techniques affected the ROS-levels in toxicological studies; 3) the applicability of different ROS detection techniques for different particles; and 4) collaboration with several other researchers in the characterisation of particle-induced ROS formation. It was found that radical detection and quantification is a challenging area and ROS measurements must be performed and used with great care. Testing three ROS probes and their response to three particle types using different particle doses showed different applicable ranges of use. This work is submitted for publication. In other work, a comparison of particle-generation of ROS with mutation frequency resulted in two publications with the Fellow as co-author. Additionally, the Fellow worked on a project to compare ROS generation and in vivo toxicity of dusts collected from a bio-fuel plant. Finally, a study on the in vitro toxicity of different types of TiO2 and their relationship with several physicochemical parameters is currently being finalized with participation of the Fellow.
Data: CORDIS, © European Union
Project objective
Given the current manufacture growth, presence of nano-particles (NP) in the environment and the future use of NP, human exposure is inevitable. Yet, the potential adverse effects on human health from exposure to NP are still largely unknown.This propos al outlines a strategy for determining how the physicochemical properties of NP influence there potential for toxicity at the National Institute of Occupational Health, Denmark and University of Copenhagen. Specifically, a wide range of NP will be characterized for their size, shape, surface area, solubility, and chemical composition.Experiments will be performed with human epithelial cells to determine the NP potential for toxicity by measuring reactive oxygen species (ROS) generation, pro-inflammatory cytokines, apoptosis, and DNA oxidative damage. By comparing the physicochemical properties of NP with their potential for toxicity, we gain the ability to predict the toxicity potential for existing and novel NP exposures.Without safety assessments and clear communication with the public and regulators, nanotechnology and the use of NP could potentially be restricted, which may hinder the economic future of Europe.This research involves the translation of physical science techniques to advance understanding of an important medical issue and mutually benefits from an ongoing and expanding nanotoxicology program at the host institute, which currently includes in vitro, in vivo and hazard evaluation aspects.My career goal is to become active in Medical Geology and Nanotoxicology. This fellowship would prove extremely valuable for my career training and serve as a springboard for future intra-Europe and cross-Atlantic collaborations.By combining several established and novel techniques from various disciplines with a well-defined research plan that is relevant to the work programme and the objectives of the European scientific programme, we are ambitious that our contribution may be significant.
Original text from CORDIS.
Participants
- NATIONAL INSTITUTE OF OCCUPATIONAL HEALTH, DENMARK · COPENHAGENCoordinatorCity levelDenmark
Links
Data: CORDIS, © European Union