ISPADEHAL · Isotopic and Proteomic Approaches to Dehalococcoides Physiology
FP7 — People (Marie Curie Actions)
- Duration
- 2009-09-01 → 2011-01-31
- EU contribution
- €161,564
- Participants
- 1
- Scheme
- MC-IEF
Lines connect the coordinator with its partners.
Results in brief
Isotopic and Proteomic Approaches to Dehalococcoides Physiology
Halogenated aromatic compounds such as highly halogenated benzenes, dioxins and biphenyls (PCBs) are toxic, persistent and widely distributed organic pollutants strongly affecting environmental quality and economical value of many grounds and aquifers throughout Europe. As found for the transformation of perchloroethene (PCE) to benign products, these highly halogenated aromatics are exclusively transformed by anaerobic bacteria of the Dehalococcoides cluster. Although Dehalococcoides species have been isolated and physiologically described, many aspects of their physiology and a procedure for easy mass cultivation remains unknown. Central research objective of the proposed project is therefore the detailed description of physiological capacities of a Dehalococcoides strain (strain CBDB1) using isotopic and proteomic technologies. Strain CBDB1 is a highly remarkable bacterium as it was the first strain for which reductive dehalogenation of chlorinated benzenes and highly toxic chlorinated dioxins was shown. In addition, several other compounds such as chlorinated phenols and chlorinated biphenyls are used as terminal electron acceptor by the strain, thereby allowing the strain to grow and reducing the toxicity of the compounds. The complete publishable summary is attached to this report.
Data: CORDIS, © European Union
Project objective
We apply for a Marie Curie Intra-European Fellowship for Dr. Ernest Marco-Urrea from Barcelona, Spain, to the group of Dr. Lorenz Adrian at the Helmholtz-Center in Leipzig, Germany. The fellowship will allow Dr. Marco-Urrea a significant expansion of his research experience which currently is focused in the aerobic degradation of halogenated ethenes by white-rot fungi. Dr. Marco-Urrea has published four referenced papers on this topic and has acquired research experience in the USA, but the next step in his career is clearly the widening of his thematic focus and of his methodological capacities in a European context. Both will be provided by the host. Dr. Marco-Urrea will continue to investigate microbial biodegradation of halogenated compounds. However, he will cultivate and perform biochemical test consistently under anaerobic conditions; he will work with halogenated aromatics rather than with aliphatics; and he will work with bacteria rather than with fungi. He will expand his already wide spectrum of techniques to the quantitative analysis of stable isotopes and to proteomic approaches. The project will complement the work that is planned in the group of Dr. Adrian on Dehalococcoides-like Chloroflexi. Dr. Marco-Urrea's main task will be the investigation of the physiological and biochemical characteristics of strain CBDB1 in an isotopic and proteomic context. Strain CBDB1 is a remarkable organism that can transform highly chlorinated dioxins, biphenyls, phenols and benzenes. It is also unique in terms of physiology, morphology and biochemistry, and its genome is fully sequenced. After an initial training phase in which isotope fractionation during growth with perchloroethene will be investigated, Dr. Marco-Urrea will use biochemical and isotopic methods to identify water-soluble electron acceptors for strain CBDB1. In a third phase, an initial proteome map under standard conditions and with alternative electron acceptors will be determined.
Original text from CORDIS.
Participants
- HELMHOLTZ-ZENTRUM FUR UMWELTFORSCHUNG GMBH - UFZ · LeipzigCoordinatorGermany
Links
Data: CORDIS, © European Union