NANOLUM · Luminescently doped nanoparticles. Strategies for improving sensitivity in luminescence assays and implementation in microarray formats
FP7 — People (Marie Curie Actions)
- Duration
- 2009-09-01 → 2012-08-31
- EU contribution
- €45,000
- Participants
- 1
- Scheme
- MC-ERG
Lines connect the coordinator with its partners.
Results in brief
Luminescently doped nanoparticles. Strategies for improving sensitivity in luminescence assays and implementation in microarray formats
The need of miniaturisation and sensitivity in (bio)chemical sensing makes luminescent-based detection methods highly appealing. The boom in nanotechnologies, together with the versatility offered by hybrid organic-inorganic materials, motivated us to explore the possibilities of different luminiscently-doped nanomaterials as optochemical sensor platforms. Different photochemical signalling mechanisms have been explored, namely Förster resonance energy transfer (FRET) coupled to ligand-to-metal charge transfer (LMCT) emission from long lived luminophores, excited state proton transfer (ESPT), photoinduced electron transfer (PET) quenching, electrogenerated chemiluminescence (ECL) and signal amplification based on plasmons generated over nanometallic surfaces. The most appropriate luminescent label for each case (boron dipyrromethenes, cyanines, ruthenium (II) polypyridyl complexes, phenazines, etc.) has been designed and synthesised. The luminescent materials were prepared mostly in nanoscaled format (core-shell nanoparticles, silica nanobeads, or nanofibres). Molecularly imprinted polymer (MIP) materials as recognition elements have also been investigated. Benchmark sensor designs have focused on the detection of fluoroquinolones, a family of antibiotics which are of widespread use in both human and veterinary medicine, becoming a health and safety concern in food and water quality control. Societal benefits from the project outcomes include those related to advanced sensor technologies, luminescent labels and functional (smart) nanomaterials. This multidisciplinary project will have an impact on the development and application of versatile sensor systems for optical detection of different chemical species of toxicological, environmental or health relevance, such as antibiotics or mycotoxins. Better detection systems, able to measure the target pollutant species in their original matrices with minimum sample treatment and lower costs, are bound to have a strong effect on the quality monitoring and control of food and water supplies. A tighter control at a reduced cost means better quality of life for the world inhabitants regardless their place of living. Contact details: Dr Ana B. Descalzo Department of Organic Chemistry Faculty of Chemistry Universidad Complutense de Madrid 28040 Madrid (Spain) Tel: +34-913-945244 E-mail: ab.descalzo@quim.ucm.es Prof. Guillermo Orellana Department of Organic Chemistry Faculty of Chemistry Universidad Complutense de Madrid 28040 Madrid (Spain) Tel: +34-913-944220 E-mail: orellana@quim.ucm.es URL: http://www.ucm.es/info/gsolfa
Data: CORDIS, © European Union
Project objective
This project aims to take advantage of polymeric nanoparticles as a tool for improving sensitivity and performance of (chemi)luminiscence based assays. For instance, highly emissive red/near-infrared (NIR) dyes and long-lifetime luminophores like Ru(II) complexes will be combined in nanoparticles for increased emission efficiencies, better discrimination from background interferences and improved photostability. These beads will be employed for labelling antibodies to be used in immunoassays. Several strategies will be explored, taking advantage of FRET processes, (chemi)luminescence of Ru(II) complexes and high emission efficiencies of red/NIR boron-dipyrromethene (BODIPY) dyes. Additionally, the use of molecularly imprinted polymers (MIPs) as synthetic analogues of antibodies will be explored for the selective recognition and fluorescent indication of analytes containing carboxylic groups. The fabrication of the MIP fluorescent probes in a nanoparticle or core-shell nanoparticle form is expected to improve response time of the sensor and binding of the target analyte, and to allow ratiometric measurements or indication via energy transfer processes. The main task will cover the synthesis of luminescent molecular probes, nanoparticles and luminescently doped nanoparticles and MIPs, with a complete physical and photophysical charaterization. These particles will be implemented in a final stage onto microarray based technologies for monitoring the presence of certain toxins and antibiotics in water and aquaculture products.
Original text from CORDIS.
Participants
- UNIVERSIDAD COMPLUTENSE DE MADRID · MadridCoordinatorSpain
Links
Data: CORDIS, © European Union