CHOLESTENOT · Study of the molecular events induced by specific functional foods with a double targeting to effectively reduce cholesterol levels
FP7 — People (Marie Curie Actions)
- Duration
- 2010-09-15 → 2012-09-14
- EU contribution
- €161,293
- Participants
- 1
- Scheme
- MC-IIF
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Results in brief
Study of the molecular events induced by specific functional foods with a double targeting to effectively reduce cholesterol levels
The aim of this project was to develop functional foods based on specific edible mushroom extracts able to effectively reduce cholesterol levels in serum by acting simultaneously at two different levels: (I) reducing endogenous cholesterol synthesis by inhibiting the hydroxymethylglutaryl‐CoA reductase (HMGCR, the key enzyme in the cholesterol metabolism) and (II) impairing exogenous cholesterol absorption during digestion by its displacement from the dietary mixed micelles, by scavenging of bile acids during the intestinal digestion to avoid their reabsorption stimulating the hepatic transformation of cholesterol bile acids and by inhibiting the pancreatic lipase (PL).
Data: CORDIS, © European Union
Project objective
This proposal is focused on the development of a meat-based functional food (pâté, sausage, snacks etc.) able to effectively reduce cholesterol levels in serum by acting simultaneously at two different levels: (I) reducing endogenous cholesterol synthesis by inhibiting the hydroxymethylglutaryl-CoA reductase (the key enzyme in the cholesterol metabolism) and (II) impairing exogenous cholesterol absorption during digestion by its displacement from the dietary mixed micelles and binding of bile acids during the intestinal digestion to avoid their reabsorption. Edible mushrooms will be submitted to accelerate solvent extraction (ASE) and supercritical fluid extraction (SFE) to obtain (T1) polysaccharides (b-glucans), (T2) statin-like or other metabolites and (T3) ergosterol-derivatives concentrates. These concentrates and their mixtures (T1+T2+T3 in a meat-based food matrix) will be screened for their properties to act as (I) and (II). All samples will be submitted to in vitro digestion and absorption tests (using Caco2 cell models) to study their bioavailability and molecular mechanism of action. Responsible compounds will be identified and quantified by analytical standardized tools (HPLC-, GC-MS etc)
Original text from CORDIS.
Participants
- UNIVERSIDAD AUTONOMA DE MADRID · MadridCoordinatorSpain
Links
Data: CORDIS, © European Union