SKELETAL STEM CELLS · Role of Skeletal Stem Cells in Bone Repair
FP7 — People (Marie Curie Actions)
- Duration
- 2010-09-01 → 2014-08-31
- EU contribution
- €100,000
- Participants
- 1
- Scheme
- MC-IRG
Lines connect the coordinator with its partners.
Results in brief
Periodic Report Summary - SKELETAL STEM CELLS (Role of Skeletal Stem Cells in Bone Repair)
The aim of the laboratory is to elucidate the cellular and molecular bases of skeletal regeneration. The skeleton exhibit high regenerative capacities that rely on the recruitment of skeletal stem cells during the early stage of bone repair shortly after bone injury. The origins of these skeletal stem cells remain elusive and most research efforts worldwide concentrate on the regenerative potentials of bone marrow-derived mesenchymal stem cells. The goal of this project is to better characterise the origins of skeletal stem cells, define the skeletal stem cells populations within adult bone and determine how these populations are established during the development and growth of the skeleton. We focus on a key source of skeletal stem cell within the periosteum, which has been shown by our group and others to largely contribute to skeletal regeneration. We have compared the origins and molecular characteristics of skeletal stem cells within periosteum compared to bone marrow, in order to better understand the bases for the high regenerative potential of periosteum-derived cells. This research will likely lead to better ways to purify skeletal stem cells for cell therapy and will allow to determine the role of skeletal stem cells within bone marrow and periosteum during bone maintenance and regeneration.
Data: CORDIS, © European Union
Project objective
It is estimated that 2% of the population sustain fractures annually, which in Europe corresponds to approximately 15 millions fractures and in France 1 million fractures. Although bone exhibits strong regenerative properties, delayed unions and non-unions still occur at a frequency of 5 to 10% in all skeletal injuries leading to patient disability and high societal cost. Bone repair is dependent on the proliferation and differentiation of skeletal stem/progenitor cells. The exact origins and functions of these cells are not clearly understood. Our long-term goal is to elucidate the mechanisms of skeletal stem cell recruitment during bone regeneration in order to support the development of new cell based therapies for bone repair. In the past 10 years, I have established several mouse models of bone repair combined with cell lineage analyses in vivo. These approaches based on genetic labeling and bone transplantation allow tracking the fate of tissues and cells during bone development and repair. In Aim 1, we will track mesenchymal lineages in development and repair, in order to understand how adult skeletal stem cell populations are established in bone. Aim 2 will directly compare the contribution of local versus systemic sources of skeletal stem cells and the effects of environmental factors such as extreme trauma on their relative contributions. Aim 3 will functionally assess the molecular regulation of skeletal cell fate decisions within bone marrow and periosteum (which lines the outer surface of bone) during fracture healing.Following my postdoctoral training, I have worked for 5 years at University of California San Francisco to develop an independent research program supported by extramural funding. This project will use my expertise accumulated over the years and research support is crucial to pursue my goals and establish a new research team at INSERM U781, Necker Hospital for Sick Children in Paris starting in the spring of 2010.
Original text from CORDIS.
Participants
- INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE · ParisCoordinatorFrance
Links
Data: CORDIS, © European Union