LCS · Study of protein dynamics in living cells after DNA damage
FP7 — People (Marie Curie Actions)
- Duration
- 2011-03-15 → 2014-03-14
- EU contribution
- €133,000
- Participants
- 2
- Scheme
- MC-IRSES
Lines connect the coordinator with its partners.
Results in brief
Study of protein dynamics in living cells after DNA damage
We declare a high quality of project realization. The goal of this project was to bring together biophysicists and biologists interested in protein kinetics and DNA damage responses. Suggested activities were guaranteed by the Institute of Biophysics Academy of Sciences of the Czech Republic and a group leader Eva Bartova, responsible for scientific goals, meeting organization and experimental strategies. All participants from Czech Republic, Russian Federation and Poland actively presented their work during their meetings and workshops, organized in the past 3 years. Many laboratory members (especially students) from the Institute in Novosibirsk and Brno started to be familiarized with possibilities of advanced microscopy techniques, including FRAP technique and FRAP analysis of protein kinetic in UV-induced DNA lesions. Research scientists and students from Novosibirsk's Institute contributed to this project by their biophysical approaches related to studies on protein kinetics. During the year 2013 we made an additional contract with Lomonosov Moscow State University in order to co-operate experimentally and organize a final meeting 2014 at this university. Our cooperation led to publication of 12 papers with acknowledgement to Marie Curie project. We published these papers in specialized scientific journals. Activities of whole scientific group are published and, for up-dated version, see: http://www.ibp.cz/cs/oddeleni/molekularni-cytologie-a-cytometrie/skupina-struktury-a-funkce-bunecneho-jadra/marie-curie-project-eu-pirses-ga-2010-269156-lcs
Data: CORDIS, © European Union
Project objective
The aim of this project is to analyze dynamics of chromatin-related proteins in living cells after induced DNA damage.Cells have developed sophisticated mechanisms to overcome damage to DNA resulting in double-strand breaks (DSBs) induced by toxic agents of our environment. Improper repair of DSBs can lead to the development of cancer. Once a cancer has developed, radiation and chemotherapy are used to damage DNA in order to kill the tumor cells. Thus, the information of how cells respond to DNA damage is critical for understanding both the development of cancer and its therapy. We want to contribute to this knowledge by the elucidation of the processes changing the chromatin structure in the DSB vicinity. Chromatin changes represent the fundamental response of the cell to DSB induction that should allow the access of repair proteins to DNA for efficient signaling and repair. It should be found how the chromatin structure is modified, what is the difference in chromatin dynamics in open and compact chromatin compartments and how are all these processes coordinated in time to allow accumulation of repair proteins for full restoration of genome integrity.The synergy of Czech expertise and the experience of Polish and Russian partners in these areas is a perfect match that will allow further advancements of science. Integration of the newest equipments and technologies together: 3D Microscopy of living cells, FISH technique, immunohistochemistry, RT-PCR and Western blotting, ChIP-PCR, ChIP-on-chips, FRAP, FLIP FRET techniques (Czech and Polish partners) and Scanning Flow Cytometry, Fluorescence Microscopy (Russian partners) should give the response to reach the aim of this project.
Original text from CORDIS.
Participants
Links
Data: CORDIS, © European Union