FTHISCOD · investigation of the histone code by high-resolution FTICR mass spectrometry and molecular biology

Линиите свързват координатора с партньорите.

Накратко на български

Цел на проекта

Using nanoflow LC coupled on-line to a novel FTICR mass spectrometer we want to describe the complexity of histone modifications and define co-occurring modifications. We want to then investigate the biological role of these simultaneous present modifications by using synthetic peptides for pull-down experiments and stable isotope labelling for quantitation and effective background subtraction to identify specifically binding proteins. We will focus after the implementation and test of the methods in a global scenario on metH3K9 which is central for gene silencing. In this way we intend to find out if other modifications are involved in fine-tuning and regulating gene silencing. This is central for our understanding of the histone code and epigenetics and may lead to a better understanding of cancer. M. Salek will move for this project to J. Rappsilber's group in Milan. He brings with him the experience of a PhD in mass spectrometric analysis of protein modifications and method development. He will be trained in LC-MS and FTICR MS and expand his experimental repertoire by peptide synthesis and pull down experiments. He will expand his analytical training into medical direction joining a campus that hosts two large cancer research institutes and is affiliated with two major cancer hospitals in Milan. The increased expertise in technology, in medical applications, and in administration will put him after this fellowship in an excellent position to respond to the European urge for proteomics groups. J. Rappsilber's group will gain with M. Salek competence essential for the proposed project and for the success of the group. M. Salek's connection to his former lab will result in transfer of expertise in the analysis of protein modification and long term access to instrumentation available there. M. Salek will go for a short stay to M. Mann's lab, one of the leading proteomics centres worldwide, and allow tightening the link to this lab while getting himself networked.

Оригинален текст от CORDIS (на английски).

Участници

• IFOM FONDAZIONE ISTITUTO FIRC ONCOLOGIA MOLECOLARE · MILANКоординаторИталия

Връзки

• Виж в CORDIS