VITANGO · DNA-based tests for commercial medicinal plant authentication
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2012-03-01 → 2016-02-29
- Финансиране от ЕС
- 449 391 €
- Участници
- 2
- Схема
- MC-IAPP
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
ДНК-тестове за лекарствени растения, като например златния корен, се разработват за разпознаване на чужд генетичен материал в препаратите. Това помага за гарантиране на чистотата на продуктите, тъй като традиционните методи за проверка често са ненадеждни.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
DNA-based tests for commercial medicinal plant authentication.
Detecting alien DNA in plant-based remedies Purity is paramount for any medicinal product. EU research is developing a DNA test for production of plant-based preparations Currently used methods for identification of plant raw material can be unreliable, especially when comminuted single plants only are available. There is a need to supplement the classically available methods that are based on pharmacognostic and phytochemical principles. The 'DNA-based tests for commercial medicinal plant authentication' (VITANGO) project – consisting of one academic and one commercial partner – is developing a sensitive quantitative test to distinguish the genetic material from that of related species. The assay can also detect low levels of any contamination from other plant species. The team used Rhodolia rosea, used in herbal medicine to fight fatigue and improve physical performance. DNA sequences have been obtained for 4 DNA barcode regions, or markers, and 10 potential adulterant species. The scientists have identified from these the most appropriate region on which to base the design of polymerase chain reaction (PCR) primers to distinguish between medicine and contaminant plants. The primers will be complementary to the DNA region targeted. More than 100 samples have gone through the assay using rosavines as chemical markers. For most samples there was agreement between the molecular and chemical identification and, where there was disparity, DNA sequencing was used to confirm PCR results. A reciprocal training scheme has been implemented between the academic and the commercial partners and will foster stronger ties and collaboration for industry and academia. Work is continuing to lower the level of contamination detectable to 2 % and to apply the methods to other medicinal plant species, including Pelargonium and black cohosh. Project deliverables promise to increase the competitive edge of European plant-based pharmaceutical products.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The unambiguous identification of plant raw material is a crucial step in the quality control of medicinal plants in order to guarantee the identity and a constant and reliable quality of the starting material. Apart from the classical pharmacognostic methods, phytochemical methods (often based on chromatography) are used for this purpose. However, these methods often fail to provide secure results, especially when only single plant parts are available, and therefore, there is a need for novel and more reliable methods. DNA-based identification methods are becoming increasingly relevant in this respect and have much potential to be powerful additions to the classical techniques.Using the medicinal plant Rhodiola rosea as an example, this project has the following objectives:a. To develop a sensitive, quantitative DNA-based test to distinguish Rhodiola rosea plant material from related plant species, and to detect low level contamination by these potential adulterantsb. To introduce the DNA test for Rhodiola authentication into the QC procedures of the commercial partnerc. To develop phytochemical and pharmacognostic methods to detect low level contamination of Rhodiola rosea plant material by potential adulterant plant speciesd. To determine the feasibility of incorporating DNA-based tests for a range of plants into the industrial partner’s quality control procedures.e. To identify the most appropriate strategy and technology platform for DNA-based tests for recommendation as an industry standard for regulation and quality assurance.These objectives will be acheived by a synergistic partnership between a university molecular biologicial research group and a phytopharmaceutical company, designed to fully exploit the opportunities for secondment and training provided by this scheme.""
Оригинален текст от CORDIS (на английски).
Участници
- DE MONTFORT UNIVERSITY · LeicesterКоординаторОбединеното кралство
- DR. WILLMAR SCHWABE GMBH & CO. KG · KARLSRUHEНиво градГермания
Връзки
Данни: CORDIS, © Европейски съюз